Prevention of familial adenomatous polyp development in APC min mice and azoxymethane-induced colon carcinogenesis in F344 Rats by ω-3 fatty acid rich perilla oil.

Abstract

The present study explored the preventive effects of perilla oil, rich in α-linolenic acid, in rodent models of colon tumorigenesis. Six-week-old male F344 rats were fed diets containing 5% corn oil or 10 or 20% perilla oil. Colonic aberrant crypt foci (ACF) were induced by azoxymethane (AOM) and colonic ACF were evaluated. In familial adenomatous polyposis mode, APC(min) mice fed with 20% corn oil or perilla oil for 80 days and intestines were evaluated for polyps. Multiple colonic mucosal and polyp samples were assayed for the expression and activity of cyclooxygenase COX-isoforms. Dietary perilla oil produced a dose-dependent inhibition of AOM-induced colonic ACF formation (by 35-53%, P < 0.01-0.005) and reduced the number of foci with ≥ 4 crypts/focus (by 38-50%, P < 0.01-0.001) in F344 rats. Dietary perilla oil significantly inhibited development of small intestinal (>69%, P < 0.0001) and colon tumors (>52%, P < 0.03) in APC(min) mice. Administration of perilla oil produced lower levels of type-2 prostaglandins (38-53%) from COX-activities in polyps of APC(min) mice. These observations demonstrate that dietary perilla oil rich in ω-3 fatty acids possesses preventive activity against intestinal neoplastic lesions, both in FAP in genetically-predisposed tissues, as well as against chemically induced preneoplastic lesions in the colon.

• Authors: Chinthalapally V Rao, Jagan M R Patlolla, Indrani Cooma, Toshihiko Kawamori, Vernon E Steele
• Journal: Nutrition and cancer (Nutr Cancer) Vol. 65 Suppl 1 Pg. 54-60 ( 2013) ISSN: 1532-7914 [Electronic] United States
• PMID: 23682783 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Anticarcinogenic Agents
• Membrane Proteins
• Plant Oils
• alpha-Linolenic Acid
• perilla seed oil
• Corn Oil
• Ptgs2 protein, mouse
• Cyclooxygenase 1
• Cyclooxygenase 2
• Ptgs1 protein, mouse
• Ptgs1 protein, rat
• Ptgs2 protein, rat
• Azoxymethane

Topics

• Aberrant Crypt Foci (chemically induced, pathology)
• Adenomatous Polyposis Coli (chemically induced, drug therapy, pathology)
• Animals
• Anticarcinogenic Agents (pharmacology)
• Azoxymethane (toxicity)
• Carcinogenesis (chemically induced, drug effects)
• Colonic Neoplasms (chemically induced, drug therapy, pathology)
• Corn Oil (administration & dosage)
• Cyclooxygenase 1 (genetics, metabolism)
• Cyclooxygenase 2 (genetics, metabolism)
• Dose-Response Relationship, Drug
• Female
• Intestinal Mucosa (drug effects, pathology)
• Male
• Membrane Proteins (genetics, metabolism)
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Plant Oils (pharmacology)
• Rats
• Rats, Inbred F344
• alpha-Linolenic Acid (pharmacology)