C1q-targeted monoclonal antibody prevents complement-dependent cytotoxicity and neuropathology in in vitro and mouse models of neuromyelitis optica.

Abstract

Neuromyelitis optica (NMO) is an autoimmune disorder with inflammatory demyelinating lesions in the central nervous system, particularly in the spinal cord and optic nerve. NMO pathogenesis is thought to involve binding of anti-aquaporin-4 (AQP4) autoantibodies to astrocytes, which causes complement-dependent cytotoxicity (CDC) and downstream inflammation leading to oligodendrocyte and neuronal injury. Vasculocentric deposition of activated complement is a prominent feature of NMO pathology. Here, we show that a neutralizing monoclonal antibody against the C1q protein in the classical complement pathway prevents AQP4 autoantibody-dependent CDC in cell cultures and NMO lesions in ex vivo spinal cord slice cultures and in mice. A monoclonal antibody against human C1q with 11 nM binding affinity prevented CDC caused by NMO patient serum in AQP4-transfected cells and primary astrocyte cultures, and prevented complement-dependent cell-mediated cytotoxicity (CDCC) produced by natural killer cells. The anti-C1q antibody prevented astrocyte damage and demyelination in mouse spinal cord slice cultures exposed to AQP4 autoantibody and human complement. In a mouse model of NMO produced by intracerebral injection of AQP4 autoantibody and human complement, the inflammatory demyelinating lesions were greatly reduced by intracerebral administration of the anti-C1q antibody. These results provide proof-of-concept for C1q-targeted monoclonal antibody therapy in NMO. Targeting of C1q inhibits the classical complement pathway directly and causes secondary inhibition of CDCC and the alternative complement pathway. As C1q-targeted therapy leaves the lectin complement activation pathway largely intact, its side-effect profile is predicted to differ from that of therapies targeting downstream complement proteins.

• Authors: Puay-Wah Phuan, Hua Zhang, Nithi Asavapanumas, Michael Leviten, Arnon Rosenthal, Lukmanee Tradtrantip, A S Verkman
• Journal: Acta neuropathologica (Acta Neuropathol) Vol. 125 Issue 6 Pg. 829-40 (Jun 2013) ISSN: 1432-0533 [Electronic] Germany
• PMID: 23677375 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Aquaporin 4
• Immunologic Factors
• Complement C1q

Topics

• Animals
• Antibodies, Monoclonal (therapeutic use)
• Aquaporin 4 (physiology)
• Cell Culture Techniques
• Complement Activation
• Complement C1q (antagonists & inhibitors)
• Cricetulus
• Disease Models, Animal
• Humans
• Immunologic Factors (therapeutic use)
• Mice
• Neuromyelitis Optica (etiology, pathology, prevention & control)