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Effect of honokiol on erythrocytes.

Abstract
Honokiol ((3,5-di-(2-propenyl)-1,1-biphenyl-2,2-diol), a component of Magnolia officinalis, stimulates apoptosis and is thus considered for the treatment of malignancy. In analogy to apoptosis of nucleated cells, erythrocytes may enter eryptosis, a suicidal death characterized by cell shrinkage and by breakdown of cell membrane phosphatidylserine asymmetry with phosphatidylserine-exposure at the erythrocyte surface. Eryptosis may be triggered following increase of cytosolic Ca(2+)-activity ([Ca(2+)]i). The present study explored, whether honokiol elicits eryptosis. Cell volume has been estimated from forward scatter, phosphatidylserine-exposure from annexin V binding, hemolysis from hemoglobin release, [Ca(2+)]i from Fluo3-fluorescence, and ceramide from fluorescent antibodies. As a result, a 48 h exposure to honokiol was followed by a slight but significant increase of [Ca(2+)]i (15 μM), significant decrease of forward scatter (5 μM), significant increase of annexin-V-binding (5 μM) and significant increase of ceramide formation (15 μM). Honokiol further induced slight, but significant hemolysis. Honokiol (15 μM) induced annexin-V-binding was significantly blunted but not abrogated in the nominal absence of extracellular Ca(2+). In conclusion, honokiol triggers suicidal erythrocyte death or eryptosis, an effect at least in part due to stimulation of Ca(2+) entry and ceramide formation.
AuthorsMohanad Zbidah, Adrian Lupescu, Tabea Herrmann, Wenting Yang, Michael Foller, Kashif Jilani, Florian Lang
JournalToxicology in vitro : an international journal published in association with BIBRA (Toxicol In Vitro) Vol. 27 Issue 6 Pg. 1737-45 (Sep 2013) ISSN: 1879-3177 [Electronic] England
PMID23673313 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Elsevier Ltd. All rights reserved.
Chemical References
  • Biphenyl Compounds
  • Lignans
  • Phosphatidylserines
  • honokiol
  • Calcium
Topics
  • Biphenyl Compounds (toxicity)
  • Calcium (metabolism)
  • Cell Death (drug effects)
  • Cell Size (drug effects)
  • Cells, Cultured
  • Erythrocytes (drug effects, metabolism, pathology)
  • Hemolysis (drug effects)
  • Humans
  • Lignans (toxicity)
  • Phosphatidylserines (metabolism)

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