Abstract | OBJECTIVE: METHOD: Twenty-two-day-old female SD rats were subcutaneously injected with 40 mg x kg(-1) N-methyl-DL-aspartic acid (NMA) at 14:00 and 16:00 every day; meanwhile, the rats were given Dabuyin Wan for intervention. Visual inspection was conducted for the time of vaginal opening. The first estrus was observed by yaginal smear test. Their ovaries and uterus were weighed to calculate organ coefficients. Conventional pathological slices were made to observe morphological changes in ovaries and uterus and calculate the thickness of uterine walls and the number of corpus luteums. The level of E2 in serum was detected to assess the therapeutic effect of Dabuyin Wan on NMA precocious puberty in rats. expressions of GnRH, GPR54 and Kiss-1 mRNA in hypothalamus were measured by semi-quantitative RT-PCR to investigate the possible mechanism of Dabuyin Wan. RESULT:
Dabuyin Wan at 3.24 g x kg(-1) and 1.62 g x kg(-1) significantly decreased the organ coefficients in rats with precocious puberty (P < 0.05), decrease the number of vaginal openings in rats (P < 0.01) and the thickness of uterine walls and the number of corpus luteums (P < 0.05), and notably down-regulated expressions of GnRH, GPR54 and Kiss-1 mRNA in hypothalamus (P < 0.05), without significant impact on E2 in serum. CONCLUSION:
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Authors | Min Cheng, Xiao-Di Ye, Yun-Ping Miao, Ai-Ying Chen, Gao-Li Zheng |
Journal | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
(Zhongguo Zhong Yao Za Zhi)
Vol. 38
Issue 3
Pg. 386-90
(Feb 2013)
ISSN: 1001-5302 [Print] China |
PMID | 23668015
(Publication Type: Journal Article)
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Chemical References |
- Drugs, Chinese Herbal
- Kiss1 protein, rat
- Kiss1r protein, rat
- Kisspeptins
- RNA, Messenger
- Receptors, G-Protein-Coupled
- Receptors, Kisspeptin-1
- dabuyin wan
- Gonadotropin-Releasing Hormone
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Topics |
- Animals
- Drugs, Chinese Herbal
(pharmacology)
- Estrus
(drug effects)
- Female
- Gene Expression
(drug effects)
- Gonadotropin-Releasing Hormone
(genetics)
- Hypothalamus
(drug effects, metabolism)
- Kisspeptins
(genetics)
- Ovary
(drug effects)
- RNA, Messenger
(genetics, metabolism)
- Rats
- Rats, Sprague-Dawley
- Receptors, G-Protein-Coupled
(genetics)
- Receptors, Kisspeptin-1
- Reverse Transcriptase Polymerase Chain Reaction
- Sexual Maturation
(drug effects, genetics)
- Time Factors
- Uterus
(drug effects)
- Vagina
(drug effects)
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