Efficacy of anti-staphylococcal protein P128 for the treatment of canine pyoderma: potential applications.

Abstract

In this study, we demonstrate the antibacterial activity of P128 on Staphylococcus isolates responsible for canine pyoderma. Eighty seven swabs were collected from dogs suffering from pyoderma and subjected to antibiotic sensitivity test and 46 Staphylococcus strains were isolated and characterized. In-vitro antimicrobial susceptibility testing with P128 was done by Minimum Inhibitory Concentration (MIC) method as per CLSI guidelines. All the Staphylococci isolated from the dogs with pyoderma, although showed resistance to various antibiotics tested, were lysed by P128. Clinical efficacy of P128 was examined in 17 dogs with pyoderma by application of the P128 hydrogel twice daily for 8 days and the results indicated complete healing of all the lesions of all the dogs under treatment. Under the conditions of this study, P128 was found to be a potent convenient proteinaceous drug for the treatment of staphylococcal pyoderma in dogs.

• Authors: Raghu Patil Junjappa, Srividya Narayanamurthy Desai, Panchali Roy, Nagalakshmi Narasimhaswamy, Juliet Roshini Mohan Raj, Murali Durgaiah, Aradhana Vipra, Udaya Ravi Bhat, Smitha Komarla Satyanarayana, Nandini Shankara, Suneelkumar Muragesh Basingi, Jagadeesh Janardhan Bhat, Sukumar Hariharan, Bharathi Sriram, Sriram Padmanabhan
• Journal: Veterinary research communications (Vet Res Commun) Vol. 37 Issue 3 Pg. 217-28 (Sep 2013) ISSN: 1573-7446 [Electronic] Netherlands
• PMID: 23661290 (Publication Type: Journal Article)

Chemical References

• P128 antistaphylococcal chimeric protein
• Recombinant Fusion Proteins

Topics

• Animals
• Dog Diseases (drug therapy, microbiology)
• Dogs
• Female
• Male
• Microbial Sensitivity Tests (veterinary)
• Polymerase Chain Reaction (veterinary)
• Polymorphism, Restriction Fragment Length
• Pyoderma (drug therapy, microbiology, veterinary)
• Recombinant Fusion Proteins (administration & dosage, therapeutic use)
• Staphylococcus (classification, drug effects, genetics, isolation & purification)