Effect of recombinant Ganoderma lucidum immunoregulatory protein on cyclophosphamide-induced leukopenia in mice.

Abstract

The aim of the study is to investigate the effect of recombinant Ganoderma lucidum immunoregulatory protein (rLZ-8) on mouse models of cyclophosphamide-induced leukopenia, which we have established with both single-phase and multi-phase administration methods. Treatment with rLZ-8 had a strong effect on both models of cyclophosphamide-induced leukopenia. In particular, it increased the number of neutrophils, lymphocytes and monocytes. rLZ-8 treatment also increased the percentage of CD4(+) T cells and the levels of secreted IL-3 and IL-4, which contributed to the cyclophosphamide-induced immune dysfunction and immune system imbalance. In conclusion, rLZ-8 treatment benefitted mice with cyclophosphamide-induced leukopenia by improving overall immune function and by specifically increasing the number of white blood cells.

• Authors: Hui Zhou, Fei Sun, Hongrui Li, Shuqin Zhang, Zhiyi Liu, Jin Pei, Chongyang Liang
• Journal: Immunopharmacology and immunotoxicology (Immunopharmacol Immunotoxicol) Vol. 35 Issue 3 Pg. 426-33 (Jun 2013) ISSN: 1532-2513 [Electronic] England
• PMID: 23656432 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Alkylating
• Cytokines
• Fungal Proteins
• LZ-8 protein, Ganoderma lucidum
• Cyclophosphamide

Topics

• Animals
• Antineoplastic Agents, Alkylating (adverse effects)
• Cyclophosphamide (adverse effects)
• Cytokines (immunology)
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay
• Flow Cytometry
• Fungal Proteins (administration & dosage, genetics, therapeutic use)
• Leukocyte Count
• Leukocytes (cytology)
• Leukopenia (blood, chemically induced, drug therapy, immunology)
• Male
• Mice
• Mice, Inbred BALB C
• Reishi (chemistry)