Serum levels and activity of indoleamine2,3-dioxygenase and tryptophanyl-tRNA synthetase and their association with disease severity in patients with chronic kidney disease.

Abstract

OBJECTIVE:

This study aims to test the serum levels and activity of indoleamine2,3-dioxygenase(IDO) and tryptophanyl-tRNA synthetase (TTS) in patients with chronic kidney disease (CKD) and to evaluate their association with disease severity.

METHOD:

Serum concentrations of IDO and TTS in 61 patients with CKD and 16 healthy volunteers were tested by ELISA. Tryptophan and kynurenine concentrations were measured by high-performance liquid chromatography (HPLC).

RESULTS:

Patients with CKD showed higher serum levels of IDO and TTS in comparison to healthy controls (p = 0.001). Patients with CKD showed lower serum levels of tryptophan and higher serum levels of kynurenine in comparison to healthy controls (p < 0.001). The kyn/Trp ratio significantly correlated with the disease severity in CKD patients (r = 0.721; p < 0.001).

CONCLUSIONS:

IDO and TTS may play critical roles in the immune pathogenesis of CKD. The activity of IDO correlated with the disease severity of CKD.

• Authors: Yu-Shi Bao, Ying Ji, Shi-Lei Zhao, Lin-Lin Ma, Ru-Juan Xie, Shi-Ping Na
• Journal: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals (Biomarkers) Vol. 18 Issue 5 Pg. 379-85 (Aug 2013) ISSN: 1366-5804 [Electronic] England
• PMID: 23651343 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• IDO1 protein, human
• IDO2 protein, human
• Indoleamine-Pyrrole 2,3,-Dioxygenase
• Kynurenine
• Tryptophan
• Tryptophan-tRNA Ligase

Topics

• Adult
• Biomarkers (blood)
• Case-Control Studies
• Female
• Glomerular Filtration Rate
• Humans
• Indoleamine-Pyrrole 2,3,-Dioxygenase (blood)
• Kynurenine (blood)
• Male
• Middle Aged
• Renal Insufficiency, Chronic (blood, enzymology, physiopathology)
• Tryptophan (blood)
• Tryptophan-tRNA Ligase (blood)