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Expression of extracellular matrix metalloproteinase inducer glycosylation and caveolin-1 in healthy and inflamed human gingiva.

AbstractBACKGROUND AND OBJECTIVE:
Glycosylated extracellular matrix metalloproteinase inducer (EMMPRIN) is specifically associated with caveolin-1 and influences its ability to induce matrix metalloproteinases (MMPs) production. This study investigated EMMPRIN glycosylation and caveolin-1 expression in healthy and inflamed human gingival tissues, analyzed the relationship between EMMPRIN glycosylation and caveolin-1 expression, and assessed how this interaction influenced MMP-1 production.
MATERIAL AND METHODS:
Gingival tissues were collected from 10 healthy subjects and 15 chronic periodontitis (chronic periodontitis) subjects. EMMPRIN, caveolin-1 and MMP-1 expressions were analyzed by immunohistochemistry. EMMPRIN and caveolin-1 co-localization was detected by immunofluorescence. EMMPRIN glycosylation, caveolin-1, active MMP-1 and proMMP-1 expression was assessed by Western blot.
RESULTS:
EMMPRIN was expressed in gingival epithelial cells, inflammatory cells, endothelial and fibroblast-like cells. Strong caveolin-1 immunoreactivity was detected in gingival epithelial and endothelial cells. Double immunofluorescence studies revealed EMMPRIN and caveolin-1 co-localization in gingival epithelium, endothelial and fibroblast-like cells. Compared with healthy subjects, the chronic periodontitis group had increased high-glycoform EMMPRIN (HG-EMMPRIN) and active MMP-1 expression (p < 0.05). Active MMP-1 and proMMP-1 protein levels were positively correlated with HG-EMMPRIN levels (p < 0.05).
CONCLUSION:
EMMPRIN and caveolin-1 colocalize in periodontal tissues. The increased active MMP-1 and proMMP-1 production may be associated with elevated HG-EMMPRIN levels.
AuthorsJ Wang, D Yang, C Li, S Shang, J Xiang
JournalJournal of periodontal research (J Periodontal Res) Vol. 49 Issue 2 Pg. 197-204 (Apr 2014) ISSN: 1600-0765 [Electronic] United States
PMID23647605 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Chemical References
  • BSG protein, human
  • Caveolin 1
  • Basigin
  • MMP1 protein, human
  • Matrix Metalloproteinase 1
Topics
  • Adolescent
  • Adult
  • Basigin (analysis)
  • Caveolin 1 (analysis)
  • Chronic Periodontitis (metabolism, pathology)
  • Dental Plaque Index
  • Endothelial Cells (chemistry)
  • Endothelium, Vascular (chemistry)
  • Epithelial Cells (chemistry)
  • Female
  • Fibroblasts (chemistry)
  • Gingiva (chemistry, cytology)
  • Glycosylation
  • Humans
  • Male
  • Matrix Metalloproteinase 1 (analysis)
  • Middle Aged
  • Periodontal Attachment Loss (classification)
  • Periodontal Index
  • Periodontal Pocket (classification)
  • Young Adult

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