Abstract | BACKGROUND: METHODS: Thirty-five CP and 27 H gingival biopsies were included. Real-time quantitative polymerase chain reaction was performed to determine mRNA levels of AIM2, DAI, and TLRs (TLR-1 through TLR-9). The difference in gene expression for each sensor between CP and H tissues was calculated using analysis of covariance. The Spearman test was used to determine correlations among innate receptors. The expression of TLR-9, AIM2, and DAI in gingival tissues was further confirmed using immunohistochemistry. RESULTS: The present results reveal statistically significant upregulation of TLR-9 (P <0.006), DAI (P <0.001), and TLR-8 (P <0.01) in CP tissues compared to H sites. Although mRNA expression was not changed significantly between groups for other receptors, the present results reveal significant correlations between receptors (P <0.05), suggesting that cooperation between multiple components of the host immune system may influence the overall response. Immunohistochemistry further confirmed expression of TLR-9, AIM2, and DAI in gingival tissues. CONCLUSIONS:
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Authors | S Esra Sahingur, Xia-Juan Xia, Stephanie C Voth, W Andrew Yeudall, John C Gunsolley |
Journal | Journal of periodontology
(J Periodontol)
Vol. 84
Issue 10
Pg. e48-57
(Oct 2013)
ISSN: 1943-3670 [Electronic] United States |
PMID | 23646855
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AIM2 protein, human
- DNA-Binding Proteins
- Nuclear Proteins
- RNA, Messenger
- RNA-Binding Proteins
- TLR3 protein, human
- TLR4 protein, human
- TLR5 protein, human
- TLR6 protein, human
- TLR7 protein, human
- TLR8 protein, human
- TLR9 protein, human
- Toll-Like Receptor 1
- Toll-Like Receptor 2
- Toll-Like Receptor 3
- Toll-Like Receptor 4
- Toll-Like Receptor 5
- Toll-Like Receptor 6
- Toll-Like Receptor 7
- Toll-Like Receptor 8
- Toll-Like Receptor 9
- Toll-Like Receptors
- ZBP1 protein, human
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Topics |
- Adult
- Aged
- Chronic Periodontitis
(immunology)
- DNA-Binding Proteins
(analysis)
- Female
- Gingiva
(immunology)
- Humans
- Male
- Melanoma
(immunology)
- Middle Aged
- Nuclear Proteins
(analysis)
- Periodontium
(immunology)
- RNA, Messenger
(analysis)
- RNA-Binding Proteins
- Real-Time Polymerase Chain Reaction
- Toll-Like Receptor 1
(analysis)
- Toll-Like Receptor 2
(analysis)
- Toll-Like Receptor 3
(analysis)
- Toll-Like Receptor 4
(analysis)
- Toll-Like Receptor 5
(analysis)
- Toll-Like Receptor 6
(analysis)
- Toll-Like Receptor 7
(analysis)
- Toll-Like Receptor 8
(analysis)
- Toll-Like Receptor 9
(analysis)
- Toll-Like Receptors
(analysis)
- Up-Regulation
- Young Adult
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