[Immunoregulation of autologous peripheral blood stem cell transplantation in patients with hepatitis B-related end-stage liver disease].

Abstract

OBJECTIVE:

To investigate the immunoregulatory effect of autologous peripheral blood stem cells (PBSCs) transplantation on T lymphocytes and cytokines in patients with HBV-related end-stage liver disease.

METHODS:

Flow cytometry was used to measure the percentages of Th1, Th2 and regulatory T cells (Treg) in peripheral blood. Enzyme-linked immunosorbent assay (ELISA) was performed to analyze the levels of serum IFN-γ, IL-6 and IL-10.

RESULTS:

Patients with HBV-related end-stage liver disease displayed significantly improved liver function after PBSCs transplantation. Statistically, after PBSCs transplantation, the percentages of Th2 and Treg in peripheral blood markedly increased, but serum IL-6 and IL-10 declined significantly. No significant differences were observed in the changes of Th1 and its cytokine, IFN-γ after transplantation.

CONCLUSION:

Autologous PBSCs transplantation can depress inflammation in liver by regulating immune microenvironment, which at least in part delineates the mechanism of stem cells-mediated therapeutic benefit on end-stage liver disease.

• Authors: Linhua Zheng, Jindong Chu, Ling Tan, Yongquan Shi, Ying Han
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 29 Issue 4 Pg. 407-10 (Apr 2013) ISSN: 1007-8738 [Print] China
• PMID: 23643171 (Publication Type: English Abstract, Journal Article)

Chemical References

• Interleukin-6
• Interleukin-10
• Interferon-gamma

Topics

• End Stage Liver Disease (immunology, virology)
• Female
• Hepatitis B (immunology)
• Humans
• Interferon-gamma (immunology)
• Interleukin-10 (immunology)
• Interleukin-6 (immunology)
• Male
• Middle Aged
• Peripheral Blood Stem Cell Transplantation (methods)
• T-Lymphocytes, Regulatory (immunology)
• Th1 Cells (immunology)
• Th2 Cells (immunology)
• Transplantation Immunology
• Transplantation, Autologous