Lower concentrations of B-vitamin subgroups in the serum and amniotic fluid correlate to cleft lip and palate appearance in the offspring of A/WySn mice.

Abstract

PURPOSE:

The pathogenesis and prevention of cleft lip and palate (CL/P) have been studied mainly in clinical and animal experiments. A prophylactic poly-B-vitamin substitution during the first months of pregnancy has provided the most encouraging results for the prevention of CL/P recurrence in families at risk. In vitro studies of the palatal organ in an A/WySn mouse model have confirmed the positive influence of B-vitamins on palatal development. The present animal study was performed to analyze different B-vitamin concentrations in the serum and amniotic fluid of A/WySn mice according to the appearance of CL/P in their offspring.

MATERIAL AND METHODS:

Concentrations of different B-vitamins (B1, B2, B3, B5, B6, and folic acid) in serum and amniotic fluid were analyzed by high-performance liquid chromatographic detection. Immunohistochemical staining against thiamin-1 receptor was performed on histologic midface sections of A/WySn fetuses with (n = 12) and without (n = 14) CL/P.

RESULTS:

Vitamin B5 (P < .001) and folic acid (P < .004) concentrations in the amniotic fluid of dams with CL/P were significantly lower than in dams without CL/P. Serum concentrations of folic acid (P = .5) and B5 (P = .4) showed no difference between the 2 groups. Dams with CL/P had significantly lower thiamine concentrations in serum (P = .01) and amniotic fluid (P < .001). Histologic midface sections presented high thiamin-1 receptor expression in the palatal shelf of fetuses with CL/P.

CONCLUSION:

A decreased use or uptake of some B-vitamin subgroups (B1, B5, and folic acid) in amniotic fluid and serum (vitamin B1) was correlated to an increased cleft appearance in A/WySn mice. The high thiamin-1 receptor expression in the palatal tissue of mouse fetuses with CL/P may be caused by a decreased availability of vitamin B1.

• Authors: Konstanze Scheller, Thomas Röckl, Christian Scheller, Johannes Schubert
• Journal: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons (J Oral Maxillofac Surg) Vol. 71 Issue 9 Pg. 1601.e1-7 (Sep 2013) ISSN: 1531-5053 [Electronic] United States
• PMID: 23642547 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

Chemical References

• Indicators and Reagents
• Membrane Transport Proteins
• Slc19a2 protein, mouse
• Vitamin B Complex
• Pantothenic Acid
• Niacinamide
• Nitroblue Tetrazolium
• Vitamin B 6
• Folic Acid
• Alkaline Phosphatase
• Adenine
• Riboflavin
• Thiamine

Topics

• Adenine (analysis, blood)
• Alkaline Phosphatase (analysis)
• Amniotic Fluid (chemistry)
• Animals
• Chromatography, High Pressure Liquid
• Cleft Lip (blood, embryology, metabolism)
• Cleft Palate (blood, embryology, metabolism)
• Disease Models, Animal
• Female
• Folic Acid (analysis, blood)
• Immunohistochemistry
• Indicators and Reagents
• Membrane Transport Proteins (analysis, blood)
• Mice
• Mice, Inbred Strains
• Niacinamide (analysis, blood)
• Nitroblue Tetrazolium
• Palate (pathology)
• Pantothenic Acid (analysis, blood)
• Pregnancy
• Riboflavin (analysis, blood)
• Thiamine (analysis, blood)
• Vitamin B 6 (analysis, blood)
• Vitamin B Complex (analysis, blood)