HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Glioma therapy using tumor homing and penetrating peptide-functionalized PEG-PLA nanoparticles loaded with paclitaxel.

Abstract
By taking advantage of the excessively upregulated expression of neuropilin (NRP) on the surface of both glioma cells and endothelial cells of angiogenic blood vessels, the ligand of NRP with high affinity - tLyp-1 peptide, which also contains a CendR motif ((R/K)XX(R/K)), was functionalized to the surface of PEG-PLA nanoparticles (tLyp-1-NP) to mediate its tumor homing, vascular extravasation and deep penetration into the glioma parenchyma. The tLyp-1-NP was prepared via a maleimide-thiol coupling reaction with uniformly spherical shape under TEM and particle size of 111.30 ± 15.64 nm. tLyp-1-NP exhibited enhanced cellular uptake in both human umbilical vein endothelial cells and Rat C6 glioma cells, increased cytotoxicity of the loaded PTX, and improved penetration and growth inhibition in avascular C6 glioma spheroids. Selective accumulation and deep penetration of tLyp-1-NP at the glioma site was confirmed by in vivo imaging and glioma distribution analysis. The longest survival was achieved by those mice bearing intracranial C6 glioma treated with PTX-loaded tLyp-1-NP. The findings here strongly indicate that tLyp-1 peptide-functionalized nanoparticulate DDS could significantly improve the efficacy of paclitaxel glioma therapy.
AuthorsQuanyin Hu, Xiaoling Gao, Guangzhi Gu, Ting Kang, Yifan Tu, Zhongyang Liu, Qingxiang Song, Lei Yao, Zhiqing Pang, Xinguo Jiang, Hongzhuan Chen, Jun Chen
JournalBiomaterials (Biomaterials) Vol. 34 Issue 22 Pg. 5640-50 (Jul 2013) ISSN: 1878-5905 [Electronic] Netherlands
PMID23639530 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Elsevier Ltd. All rights reserved.
Chemical References
  • Cell-Penetrating Peptides
  • Coumarins
  • monomethoxypolyethyleneglycol-polylactide block copolymer
  • Polyethylene Glycols
  • coumarin
  • Paclitaxel
Topics
  • Animals
  • Apoptosis (drug effects)
  • Cell Line, Tumor
  • Cell Proliferation (drug effects)
  • Cell Shape (drug effects)
  • Cell Survival (drug effects)
  • Cell-Penetrating Peptides (pharmacology)
  • Coumarins (metabolism)
  • Glioma (drug therapy, pathology)
  • Human Umbilical Vein Endothelial Cells (drug effects, metabolism)
  • Humans
  • Kaplan-Meier Estimate
  • Male
  • Mice
  • Mice, Nude
  • Nanoparticles (chemistry, ultrastructure)
  • Paclitaxel (pharmacology, therapeutic use)
  • Polyethylene Glycols (chemistry)
  • Rats
  • Spectroscopy, Near-Infrared
  • Spheroids, Cellular (drug effects, pathology)
  • Tissue Distribution (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: