Abstract |
Trogocytosis is a contact-dependent intercellular transfer of membrane fragments and associated molecules from APCs to effector lymphocytes. We previously demonstrated that trogocytosis also occurs between tumor target and cognate melanoma Ag-specific cytotoxic T cells (CTL). In this study, we show that, following trogocytosis, immune effector cells acquire molecular components of the tumor, including surface Ags, which are detectable by specific mAbs. We demonstrate that CD8(+) and CD4(+) T cells from melanoma patients' PBMC and tumor-infiltrating lymphocytes (TIL) capture melanoma Ags, enabling identification of trogocytosing lymphocytes by staining with Ag-specific Abs. This finding circumvents the necessity of tumor prelabeling, which in the past was mandatory to detect membrane-capturing T cells. Through the detection of melanoma Ags on TIL, we sorted trogocytosing T cells and verified their preferential reactivity and cytotoxicity. Furthermore, tumor Ag-imprinted T cells were detected at low frequency in fresh TIL cultures shortly after extraction from the tumor. Thus, T cell imprinting by tumor Ags may allow the enrichment of melanoma Ag-specific T cells for research and potentially even for the adoptive immunotherapy of patients with cancer.
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Authors | Galit Eisenberg, Ronny Uzana, Aviad Pato, Shoshana Frankenburg, Sharon Merims, Eitan Yefenof, Soldano Ferrone, Tamar Peretz, Arthur Machlenkin, Michal Lotem |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 190
Issue 11
Pg. 5856-65
(Jun 01 2013)
ISSN: 1550-6606 [Electronic] United States |
PMID | 23626012
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- HLA-A Antigens
- Melanoma-Specific Antigens
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Topics |
- Cell Line, Tumor
- HLA-A Antigens
(chemistry, immunology)
- Humans
- Leukocytes, Mononuclear
(immunology, metabolism)
- Lymphocytes, Tumor-Infiltrating
(immunology, metabolism)
- Melanoma
(immunology, metabolism)
- Melanoma-Specific Antigens
(chemistry, immunology)
- T-Lymphocyte Subsets
(immunology, metabolism)
- T-Lymphocytes
(immunology, metabolism)
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