Abstract |
A high throughput method was developed for rapid screening and identification of bioactive compounds from traditional Chinese medicine, marine products and other natural products. The system, integrated with five-channel chromatographic separation and dual UV-MS detection, is compatible with in vitro 96-well microplate based bioassays. The stability and applicability of the proposed method was validated by testing radical scavenging capability of a mixture of seven known compounds ( rutin, dihydroquercetin, salvianolic acid A, salvianolic acid B, glycyrrhizic acid, rubescensin A and tangeretin). Moreover, the proposed method was successfully applied to the crude extracts of traditional Chinese medicine and a marine sponge from which 12 bioactive compounds were screened and characterized based on their anti-oxidative or anti- tumor activities. In particular, two diterpenoid derivatives, agelasine B and (-)- agelasine D, were identified for the first time as anti- tumor compounds from the sponge Agelas mauritiana, showing a considerable activity toward MCF-7 cells (IC50 values of 7.84±0.65 and 10.48±0.84 μM, respectively). Our findings suggested that the integrated system of 5-channel parallel chromatography coupled with on-line mass spectrometry and microplate based assays can be a versatile and high efficient approach for the discovery of active compounds from natural products.
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Authors | Yufeng Zhang, Shun Xiao, Lijuan Sun, Zhiwei Ge, Fengkai Fang, Wen Zhang, Yi Wang, Yiyu Cheng |
Journal | Analytica chimica acta
(Anal Chim Acta)
Vol. 777
Pg. 49-56
(May 13 2013)
ISSN: 1873-4324 [Electronic] Netherlands |
PMID | 23622964
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Validation Study)
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Copyright | Copyright © 2013 Elsevier B.V. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- Antioxidants
- Biological Products
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Topics |
- Animals
- Antineoplastic Agents
(chemistry, pharmacology)
- Antioxidants
(chemistry, pharmacology)
- Biological Assay
(methods)
- Biological Products
(chemistry, pharmacology)
- Cell Line, Tumor
- Chromatography, Liquid
- Drug Screening Assays, Antitumor
- Drug Stability
- HL-60 Cells
- Humans
- Mass Spectrometry
- Porifera
(chemistry)
- Reproducibility of Results
- Time Factors
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