CCCTC-binding factor controls its own nuclear transport via regulating the expression of importin 13.

Abstract

CCCTC-binding factor (CTCF), a multivalent zinc-finger protein, is involved in different aspects of regulation including promoter activation or repression, gene silencing, chromatin insulation, gene imprinting, X-chromosome inactivation, cell growth or differentiation and tumor genesis. However, the molecular mechanisms of CTCF nuclear import remains unclear. In this study, we showed that the expression of CTCF influenced the intracellular distribution of itself, which might go through transport receptor - import 13 (IPO13). We further confirmed that there is a CTCF target site in ipo13 -774∼-573 bp promoter region and CTCF regulates the expression of IPO13. Besides, GST pull-down and Co-IP experiments demonstrated that CTCF interacts with IPO13. Immunofluorescence staining showed that IPO13 influenced intracellular distribution of CTCF. In all, we conclude that CTCF regulates the expression of IPO13, which, in turn, mediates the nuclear import of CTCF.

• Authors: Rong Wang, Jingjing Shen, Peitang Huang, Xudong Zhu
• Journal: Molecules and cells (Mol Cells) Vol. 35 Issue 5 Pg. 388-95 (May 2013) ISSN: 0219-1032 [Electronic] Korea (South)
• PMID: 23620300 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCCTC-Binding Factor
• CTCF protein, human
• Chromatin
• IPO13 protein, human
• Karyopherins
• Repressor Proteins

Topics

• Active Transport, Cell Nucleus
• CCCTC-Binding Factor
• Cell Line, Tumor
• Chromatin (metabolism)
• Fluorescent Antibody Technique
• Gene Silencing
• Humans
• Karyopherins (genetics, metabolism)
• Promoter Regions, Genetic
• Repressor Proteins (metabolism)