Characterization, purification of Poncirin from edible citrus Ougan (Citrus reticulate cv. Suavissima) and its growth inhibitory effect on human gastric cancer cells SGC-7901.

Abstract

Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit (1.37 mg/g DW). High speed counter-current chromatography (HSCCC) combined with D101 resin chromatography was utilized for the separation and purification of poncirin from the albedo of Ougan fruit. After this two-step purification, poncirin purity increased from 0.14% to 96.56%. The chemical structure of the purified poncirin was identified by both HPLC-PDA and LC-MS. Poncirin showed a significant in vitro inhibitory effect on the growth of the human gastric cancer cells, SGC-7901, in a dose-dependent manner. Thus, poncirin from Ougan fruit, may be beneficial for gastric cancer prevention. The purification method demonstrated here will be useful for further studies on the pharmacological mechanism of poncirin activity, as well as for guiding the consumption of Ougan fruit.

• Authors: Xiaoyan Zhu, Fenglei Luo, Yixiong Zheng, Jiukai Zhang, Jianzhen Huang, Chongde Sun, Xian Li, Kunsong Chen
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 14 Issue 5 Pg. 8684-97 (Apr 24 2013) ISSN: 1422-0067 [Print] Switzerland
• PMID: 23615464 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Flavonoids
• Solvents
• poncirin

Topics

• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Chromatography, High Pressure Liquid
• Citrus (chemistry)
• Countercurrent Distribution
• Drug Screening Assays, Antitumor
• Flavonoids (chemistry, isolation & purification, pharmacology)
• Humans
• Solvents
• Spectrophotometry, Ultraviolet
• Stomach Neoplasms (pathology)