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The endoplasmic reticulum is a reservoir for WAVE/SCAR regulatory complex signaling in the Arabidopsis leaf.

Abstract
During plant cell morphogenesis, signal transduction and cytoskeletal dynamics interact to locally organize the cytoplasm and define the geometry of cell expansion. The WAVE/SCAR (for WASP family verprolin homologous/suppressor of cyclic AMP receptor) regulatory complex (W/SRC) is an evolutionarily conserved heteromeric protein complex. Within the plant kingdom W/SRC is a broadly used effector that converts Rho-of-Plants (ROP)/Rac small GTPase signals into Actin-Related Protein2/3 and actin-dependent growth responses. Although the components and biochemistry of the W/SRC pathway are well understood, a basic understanding of how cells partition W/SRC into active and inactive pools is lacking. In this paper, we report that the endoplasmic reticulum (ER) is an important organelle for W/SRC regulation. We determined that a large intracellular pool of the core W/SRC subunit NAP1, like the known positive regulator of W/SRC, the DOCK family guanine nucleotide-exchange factor SPIKE1 (SPK1), localizes to the surface of the ER. The ER-associated NAP1 is inactive because it displays little colocalization with the actin network, and ER localization requires neither activating signals from SPK1 nor a physical association with its W/SRC-binding partner, SRA1. Our results indicate that in Arabidopsis (Arabidopsis thaliana) leaf pavement cells and trichomes, the ER is a reservoir for W/SRC signaling and may have a key role in the early steps of W/SRC assembly and/or activation.
AuthorsChunhua Zhang, Eileen Mallery, Sara Reagan, Vitaly P Boyko, Simeon O Kotchoni, Daniel B Szymanski
JournalPlant physiology (Plant Physiol) Vol. 162 Issue 2 Pg. 689-706 (Jun 2013) ISSN: 1532-2548 [Electronic] United States
PMID23613272 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Actins
  • Arabidopsis Proteins
  • DIS3 protein, Arabidopsis
  • Multiprotein Complexes
  • SPIKE1 protein, Arabidopsis
  • Adenosine Triphosphatases
  • NAP1 protein, Arabidopsis
Topics
  • Actins (metabolism)
  • Adenosine Triphosphatases (genetics, metabolism)
  • Arabidopsis (genetics, metabolism)
  • Arabidopsis Proteins (genetics, metabolism)
  • Endoplasmic Reticulum (genetics, metabolism)
  • Intracellular Membranes (metabolism)
  • Multiprotein Complexes (metabolism)
  • Plant Leaves (genetics, metabolism)
  • Plants, Genetically Modified
  • Signal Transduction
  • Trichomes (metabolism)

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