Wogonoside displays anti-inflammatory effects through modulating inflammatory mediator expression using RAW264.7 cells.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

The root of Scutellaria baicalensis Georgi, also called Huangqin in China, is an herbal-based nutraceutical which is usually used in Chinese medicated diet (CMD). As an abundant ingredient in Huangqin, wogonoside is a flavonoid glycoside. The present work investigated the anti-inflammatory activities of wogonoside in lipopolysaccharides (LPS)-induced RAW264.7 cells.

MATERIALS AND METHODS:

RAW264.7 cells were used. The inhibition of wogonoside against nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in LPS-induced RAW264.7 cells were measured. Additionally, the effects of wogonoside on mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX2), TNF-α and IL-6 were also investigated.

RESULTS AND DISCUSSION:

Wogonoside not only dose-dependently decreased the production of inflammatory mediators including NO and PGE2 but also inhibited the release of pro-inflammatory cytokines including TNF-α and IL-6 in LPS-induced RAW264.7 cells. Furthermore, wogonoside possessed significantly in vitro inhibitory effects on the gene expression of iNOS, COX2, TNF-α and IL-6.

CONCLUSION:

These results suggest that wogonoside may be used as a functional food component for prevention and treatment of inflammation.

• Authors: Yao-Zhi Yang, You-Zhi Tang, Ya-Hong Liu
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 148 Issue 1 Pg. 271-6 (Jun 21 2013) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 23612420 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Flavanones
• Glucosides
• Interleukin-6
• Lipopolysaccharides
• Tumor Necrosis Factor-alpha
• Nitric Oxide
• Nitric Oxide Synthase Type II
• Nos2 protein, mouse
• Ptgs2 protein, mouse
• Cyclooxygenase 2
• wogonoside
• Dinoprostone

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cell Line
• Cell Survival (drug effects)
• Cyclooxygenase 2 (genetics)
• Dinoprostone (metabolism)
• Flavanones (pharmacology)
• Glucosides (pharmacology)
• Interleukin-6 (genetics, metabolism)
• Lipopolysaccharides
• Mice
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type II (genetics)
• Tumor Necrosis Factor-alpha (genetics, metabolism)