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Serological investigation of highly pathogenic avian influenza H5N2 in ostriches (Struthio camelus).

Abstract
An ostrich farm of 929 birds that tested polymerase chain reaction-positive for highly pathogenic avian influenza H5N2 in a single sample was designated for culling, despite no evidence of sero-conversion as assessed by haemagglutination inhibition (HI) tests. A month later and immediately prior to culling, all birds were bled and tested with an IDEXX avian influenza virus (AIV) nucleoprotein (NP)-specific enzyme-linked immunosorbent assay (ELISA) and a high sero-prevalence was detected. To address the question of whether the NP-specific antibodies detected indicated exposure to H5 or non-H5 subtypes (H6N2 and H1N2 strains were also circulating regionally at the time), we developed two H5-specific ELISAs, both based on a recombinant H5 HA1 antigen. The H5 indirect ELISA used a horseradish peroxidase ostrich IgY conjugate that we produced in chicken eggs. The single-chain variable fragment (scFv) competitive ELISA (H5 scFv cELISA) used a scFv derived from an H5-immune chicken scFv library. By comparing IDEXX AIV ELISA results with those of the two H5-specific ELISAs and HI tests, we determined that up to 89% of the flock had been exposed to H5N2 AIV. We also detected evidence of suspected vaccination, since 17% of sera contained antibodies against the H5 glycoprotein but not the NP protein. Comparative analytical sensitivity indicated that HI tests are likely to miss up to 35% of H5-positive samples, and thus we consider that H5/H7-specific ELISAs should replace HI tests for ostrich testing in future.
AuthorsCelia Abolnik, Jeanni Fehrsen, Adriaan Olivier, Wouter van Wyngaardt, Geoffrey Fosgate, Charlotte Ellis
JournalAvian pathology : journal of the W.V.P.A (Avian Pathol) Vol. 42 Issue 3 Pg. 206-14 ( 2013) ISSN: 1465-3338 [Electronic] England
PMID23607480 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Hemagglutinin Glycoproteins, Influenza Virus
  • hemagglutinin, avian influenza A virus
Topics
  • Animals
  • Enzyme-Linked Immunosorbent Assay (methods, veterinary)
  • Hemagglutination Tests (veterinary)
  • Hemagglutinin Glycoproteins, Influenza Virus (genetics)
  • Influenza A Virus, H5N2 Subtype (genetics)
  • Influenza in Birds (epidemiology, virology)
  • Sensitivity and Specificity
  • Seroepidemiologic Studies
  • South Africa
  • Struthioniformes

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