Combinational effect of PPARγ agonist and RXR agonist on the growth of SGC7901 gastric carcinoma cells in vitro.

Abstract

In order to investigate the inhibitory effects and mechanisms of troglitazone (TGZ), a peroxisome proliferator-activated receptor γ (PPARγ) agonist, and retinoid X receptor (RXR) agonist (9-cis-retinoic acid (RA)) on gastric carcinoma cells SGC7901, SGC7901 cells were treated with TGZ and 9-cis-RA, respectively, or in combination. Then, the cell growth, apoptosis, morphological changes, and the expression of PPARγ, RXRγ, Bcl-2, and Bax were detected by MTT assay, flow cytometry, HE staining, immunocytochemistry staining, and Western blot assay, respectively. Our results showed that the growth of SGC7901 cells was inhibited and the cells got sparser at the concentrations of 50 μmol/L TGZ, 20 μmol/L 9-cis-RA, or combination of TGZ (25 μmol/L) and 9-cis-RA (10 μmol/L). Immunocytochemistry and Western blot showed that after 72 h, the expression of PPARγ, RXRγ, and Bax were upregulated; Bcl-2 was downregulated compared with the negative control group. These data indicated that PPARγ agonist and RXR agonist could inhibit the proliferation of SGC7901 cells via inducing the apoptosis, which involved the increase in the level of Bax/Bcl-2. The combination of RXR agonist and PPARγ agonist could induce the maximal inhibitory effects on tumor growth and apoptosis via promoting the formation of RXR/PPARγ heterodimer.

• Authors: Ying Liu, Zu-an Zhu, Shang-Nuan Zhang, Jie Mou, Lei Liu, Tao Cui, Dong-Sheng Pei
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 34 Issue 4 Pg. 2409-18 (Aug 2013) ISSN: 1423-0380 [Electronic] Netherlands
• PMID: 23605322 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Chromans
• Hypoglycemic Agents
• Intercalating Agents
• PPAR gamma
• Proto-Oncogene Proteins c-bcl-2
• Retinoid X Receptors
• Thiazolidinediones
• bcl-2-Associated X Protein
• Alitretinoin
• Propidium
• Tretinoin
• Troglitazone

Topics

• Alitretinoin
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Chromans (pharmacology)
• Down-Regulation
• Humans
• Hypoglycemic Agents (pharmacology)
• Intercalating Agents (pharmacology)
• PPAR gamma (agonists, biosynthesis)
• Propidium (pharmacology)
• Proto-Oncogene Proteins c-bcl-2 (biosynthesis)
• Retinoid X Receptors (agonists, biosynthesis)
• Stomach Neoplasms (drug therapy)
• Thiazolidinediones (pharmacology)
• Tretinoin (pharmacology)
• Troglitazone
• Up-Regulation
• bcl-2-Associated X Protein (biosynthesis)