Characterization of the promoter region of an Arabidopsis gene for 9-cis-epoxycarotenoid dioxygenase involved in dehydration-inducible transcription.

Abstract

Plants respond to dehydration stress and tolerate water-deficit status through complex physiological and cellular processes. Many genes are induced by water deficit. Abscisic acid (ABA) plays important roles in tolerance to dehydration stress by inducing many stress genes. ABA is synthesized de novo in response to dehydration. Most of the genes involved in ABA biosynthesis have been identified, and they are expressed mainly in leaf vascular tissues. Of the products of such genes, 9-cis-epoxycarotenoid dioxygenase (NCED) is a key enzyme in ABA biosynthesis. One of the five NCED genes in Arabidopsis, AtNCED3, is significantly induced by dehydration. To understand the regulatory mechanism of the early stages of the dehydration stress response, it is important to analyse the transcriptional regulatory systems of AtNCED3. In the present study, we found that an overlapping G-box recognition sequence (5'-CACGTG-3') at -2248 bp from the transcriptional start site of AtNCED3 is an important cis-acting element in the induction of the dehydration response. We discuss the possible transcriptional regulatory system of dehydration-responsive AtNCED3 expression, and how this may control the level of ABA under water-deficit conditions.

• Authors: Babak Behnam, Satoshi Iuchi, Miki Fujita, Yasunari Fujita, Hironori Takasaki, Yuriko Osakabe, Kazuko Yamaguchi-Shinozaki, Masatomo Kobayashi, Kazuo Shinozaki
• Journal: DNA research : an international journal for rapid publication of reports on genes and genomes (DNA Res) Vol. 20 Issue 4 Pg. 315-24 (Aug 2013) ISSN: 1756-1663 [Electronic] England
• PMID: 23604098 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plant Proteins
• Water
• Abscisic Acid
• Dioxygenases
• 9-cis-epoxy-carotenoid dioxygenase

Topics

• Abscisic Acid (biosynthesis, genetics)
• Arabidopsis (genetics, metabolism)
• Base Sequence
• Dehydration
• Dioxygenases (genetics, metabolism)
• Gene Expression Regulation, Plant
• Molecular Sequence Data
• Plant Leaves (genetics, metabolism)
• Plant Proteins (genetics, metabolism)
• Promoter Regions, Genetic
• Stress, Physiological
• Transcription, Genetic
• Water (metabolism)