Combination treatment with platycodin D and osthole inhibits cell proliferation and invasion in mammary carcinoma cell lines.

Abstract

In this study, two invasive mammary carcinoma cells (MDA-MB-231 and 4T1) were utilized to evaluate the inhibitory activities of platycodin D, osthole, and the two in combination. The anti-proliferative effect was tested using the MTT and BrdU assay, and the combination of 15μM osthole and 75μM platycodin D was used for subsequent analyses. The anti-invasive effect was evaluated by the transwell assay. The results showed that the combination treatment reduced both cell proliferation and invasion. Western blot and real-time PCR revealed that the platycodin D-osthole combination significantly decreased TβRII, Smad2, Smad3 and Smad4 gene or protein expressions, as well as effectively blocked TGF-β-induced phosphorylation of Smad2 and Smad3. Thus, this study demonstrates that the anti-cancer effects of the platycodin D-osthole combination in breast cancer cells involve proliferation inhibition and invasion blockade, both of which may be mediated by perturbations in the TGF-β/Smads pathway.

• Authors: Yiyi Ye, Xianghui Han, Baofeng Guo, Zhenping Sun, Sheng Liu
• Journal: Environmental toxicology and pharmacology (Environ Toxicol Pharmacol) Vol. 36 Issue 1 Pg. 115-24 (Jul 2013) ISSN: 1872-7077 [Electronic] Netherlands
• PMID: 23603464 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Chemical References

• Antineoplastic Agents
• Coumarins
• Receptors, Transforming Growth Factor beta
• Saponins
• Smad Proteins
• Transforming Growth Factor beta
• Triterpenes
• platycodin D
• Protein Serine-Threonine Kinases
• Receptor, Transforming Growth Factor-beta Type II
• osthol

Topics

• Animals
• Antineoplastic Agents (administration & dosage)
• Breast Neoplasms (drug therapy, genetics, metabolism)
• Carcinoma (drug therapy, genetics, metabolism)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Coumarins (administration & dosage)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Mice
• Protein Serine-Threonine Kinases (genetics, metabolism)
• Receptor, Transforming Growth Factor-beta Type II
• Receptors, Transforming Growth Factor beta (genetics, metabolism)
• Saponins (administration & dosage)
• Smad Proteins (genetics, metabolism)
• Transforming Growth Factor beta (pharmacology)
• Triterpenes (administration & dosage)