A prostaglandin D2 metabolite is elevated in the urine of Duchenne muscular dystrophy patients and increases further from 8 years old.

Abstract

BACKGROUND:

Duchenne muscular dystrophy (DMD) is a progressive muscle wasting disease caused by muscle dystrophin deficiency. Downstream of the primary dystrophin deficiency is not well elucidated. Here, the hypothesis that prostaglandin D2 (PGD2)-mediated inflammation is involved in the pathology of DMD was examined by measuring tetranor PGDM, a major PGD2 metabolite, in urine of DMD patients.

METHODS:

We measured tetranor PGDM in urine using LC-MS/MS. First morning urine samples were collected from genetically confirmed DMD patients and age-matched healthy controls aged 4 to 15y.

RESULTS:

The urinary tetranor PGDM concentration was 3.08±0.15 and 6.90±0.35ng/mg creatinine (mean±SE) in 79 control and 191 DMD samples, respectively. The mean concentration was approximately 2.2-times higher in DMD patients than in controls (p<0.05). Remarkably, urinary tetranor PGDM concentrations in DMD patients showed chronological changes: it stayed nearly 1.5 times higher than in controls until 7y but surged at the age of 8y to a significantly higher concentration.

CONCLUSION:

Urinary tetranor PGDM concentrations were shown to be increased in DMD patients and became higher with advancing age. It was indicated that PGD2-mediated inflammation plays a role in the pathology of DMD.

• Authors: Taku Nakagawa, Atsuko Takeuchi, Ryohei Kakiuchi, Tomoko Lee, Mariko Yagi, Hiroyuki Awano, Kazumoto Iijima, Yasuhiro Takeshima, Yoshihiro Urade, Masafumi Matsuo
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 423 Pg. 10-4 (Aug 23 2013) ISSN: 1873-3492 [Electronic] Netherlands
• PMID: 23603101 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Chemical References

• Prostaglandin D2

Topics

• Adolescent
• Age Factors
• Child
• Child, Preschool
• Chromatography, Liquid
• Humans
• Muscular Dystrophy, Duchenne (pathology, urine)
• Prostaglandin D2 (urine)
• Tandem Mass Spectrometry