HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Associations between pigment epithelium-derived factor, insulin resistance and high density lipoprotein.

AbstractAIMS:
To measure serum pigment epithelium-derived factor in control subjects with normal fasting glucose, and in subjects with impaired fasting glucose and those with newly diagnosed Type 2 diabetes, before treatment initiation, and to measure pigment epithelium-derived factor prospectively in patients being treated with HDL-raising therapy, niacin.
METHODS:
We enrolled 89 individuals attending an institutional health screen. Biochemical indices including lipids, homeostasis model assessment-insulin resistance, high-sensitivity C-reactive protein and pigment epithelium-derived factor were analysed in fasting blood. To validate the association between HDL and pigment epithelium-derived factor, we analysed samples from a separate study cohort with low HDL, followed up for 12-weeks while on niacin treatment. Secreted pigment epithelium-derived factor from 3T3-L1 adipocytes, after HDL treatment (24-h), was measured using Western blot analysis.
RESULTS:
Mean (± sd) serum pigment epithelium-derived factor was significantly higher in subjects with impaired fasting glucose [13.99 (± 3.06) μg/ml] and Type 2 diabetes [12.94 (± 2.61)] μg/ml, compared with control subjects [11.83 (± 2.85) μg/ml (P = 0.014)]. In multivariate analyses, serum pigment epithelium-derived factor concentration was associated with BMI (β = 0.32, 0.007), homeostasis model assessment-insulin resistance (β = 0.33, P = 0.01) and HDL (β = -0.24, P = 0.05), after adjustment for age, gender and high-sensitivity C-reactive protein. In the niacin study, on-treatment HDL was an independent determinant of pigment epithelium-derived factor (β = -0.439, P = 0.033), after adjusting for age, homeostasis model assessment-insulin resistance and treatment. Adipocytes treated with HDL were found to have reduced pigment epithelium-derived factor secretion [24.8% (50 μg/ml), 28.4% (100 μg/ml) HDL; P < 0.05)], compared with the control samples.
CONCLUSION:
Serum pigment epithelium-derived factor is positively associated with homeostasis model assessment-insulin resistance and negatively associated with HDL. Further studies are needed to understand the mechanism of low HDL and raised pigment epithelium-derived factor and to determine if they are causally related to the pathobiology of insulin resistance.
AuthorsS Pek, S Tavintharan, K Woon, J Niyati, S C Lim, C F Sum
JournalDiabetic medicine : a journal of the British Diabetic Association (Diabet Med) Vol. 30 Issue 9 Pg. 1067-74 (Sep 2013) ISSN: 1464-5491 [Electronic] England
PMID23600479 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2013 The Authors. Diabetic Medicine © 2013 Diabetes UK.
Chemical References
  • Eye Proteins
  • Lipoproteins, HDL
  • Nerve Growth Factors
  • Serpins
  • pigment epithelium-derived factor
Topics
  • 3T3-L1 Cells
  • Adipocytes, White (metabolism, secretion)
  • Adolescent
  • Adult
  • Aged
  • Animals
  • Body Mass Index
  • Cohort Studies
  • Cross-Sectional Studies
  • Diabetes Mellitus, Type 2 (blood, complications, metabolism)
  • Eye Proteins (blood, secretion)
  • Female
  • Follow-Up Studies
  • Humans
  • Insulin Resistance
  • Lipoproteins, HDL (blood, metabolism)
  • Male
  • Mice
  • Middle Aged
  • Nerve Growth Factors (blood, secretion)
  • Overweight (complications)
  • Prediabetic State (blood, complications, metabolism)
  • Prospective Studies
  • Serpins (blood, secretion)
  • Young Adult

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: