Abstract | OBJECTIVE: METHODS: RT-PCR, real-time quantitative PCR (Q-PCR)and in situ hybridization (ISH) were used to detect the expression of Bfl-1 mRNA in prostate cancer cell lines, tissues and benign prostate hyperplasia (BPH) tissue samples. The relationship between Bfl-1 expression and clinicopathological parameters was analyzed. Antisense oligonucleotides (ASONs) were used to interfere the expression of Bfl-1 and its effects on prostate cancer cells. MTT was used to detect the survival, morphologic changes of prostate cancer cells was observed by inverted microscope. RESULTS: Bfl-1 mRNA, detected by RT-PCR, Q-PCR and ISH, was overexpressed in the androgen-independent prostate cancer cell lines PC-3 and DU145, but not detectable in the androgen-dependent prostate cancer cell line LNCaP and BPH tissue samples (P < 0.05). Significantly higher Bfl-1 mRNA levels were observed in higher stage and metastatic prostate cancer cases than those without metastasis or of low stage. ASONs targeting Bfl-1 significantly inhibited androgen-independent prostate cancer cell growth (P < 0.05), cell was rounding off or fragmentation. CONCLUSION:
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Authors | Xing-Lan Li, Xue-Qin Chen, Ling Nie, Miao Xu, Qiu-Yao Li, Wei-Wei Shang, Ni Chen, Rui Huang, Hao Zeng, Qiao Zhou |
Journal | Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
(Sichuan Da Xue Xue Bao Yi Xue Ban)
Vol. 44
Issue 1
Pg. 21-6
(Jan 2013)
ISSN: 1672-173X [Print] China |
PMID | 23600202
(Publication Type: Journal Article)
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Chemical References |
- BCL2-related protein A1
- Minor Histocompatibility Antigens
- Oligonucleotides, Antisense
- Proto-Oncogene Proteins c-bcl-2
- RNA, Messenger
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Topics |
- Apoptosis
- Cell Line
- Cell Transformation, Neoplastic
- Humans
- In Situ Hybridization
- Male
- Minor Histocompatibility Antigens
- Oligonucleotides, Antisense
- Prostatic Hyperplasia
(metabolism)
- Prostatic Neoplasms
(metabolism)
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- RNA, Messenger
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