[The effect of eukaryotic expression plasmid ARHI on gastric cancer].

Abstract

OBJECTIVE:

To study the effect of expressed aplasia ras homolog member I (ARHI) on the malignant biological behaviors of gastric cancer including the proliferation, migration and invasion of the cell.

METHODS:

The eukaryotic expression plasmid of ARHI was constructed and transfected into MKN-28 cell with lipofectamine 2000 as pEGFP-ARHI group, transfected with pEGFP-N1 as pEGFP-N1 group, and untreated MKN-28 as control group. The expression of ARHI was detected by Western blotting and fluorescence microscope. CCK-8 assay was used to analyze the cell proliferation, the wound-healing assay and transwell assay were performed to investigate the effects on migration and invasion.

RESULTS:

Compared with the pEGFP-N1 group and control group, proliferation, invasion and migration of the pEGFP-ARHI group were depressed (P < 0.05).

CONCLUSION:

Recombination eukaryotic expression pEGFP-ARHI could partially reverse the malignant phenotypes of gastric cancer cell MKN-28.

• Authors: Li-Bo Li, Qiu-Lin Tang, Ming-Xing Li, Wei-Bing Leng, Liu Chen, Dan-Dan Yuan, Rong-Hui Li, Feng Bi
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 44 Issue 1 Pg. 10-4, 20 (Jan 2013) ISSN: 1672-173X [Print] China
• PMID: 23600200 (Publication Type: Journal Article)

Chemical References

• DIRAS3 protein, human
• rho GTP-Binding Proteins

Topics

• Cell Line, Tumor
• Cell Proliferation
• Humans
• Plasmids
• Stomach Neoplasms
• Transfection
• rho GTP-Binding Proteins (genetics)