Abstract |
A method for quantitation of D-glucaric acid in bile has been developed involving extraction with tetrahexylammonium chloride, boiling for 40-60 min, and determination of the percentage inhibition of beta-glucuronidase activity at 56 degrees C and pH 4. D-glucaric acid, bilirubin, bile acid, and protein were determined in 106 human gallbladder biles obtained at autopsy, including 20 with gallstones. The mean D-glucaric acid content was 1125 +/- 159 microM (mean +/- SE). Biliary beta-glucuronidase activity was not affected by D-glucaric acid because of 1) no difference in biliary D-glucaric acid content, either absolute or corrected for per unit of bilirubin, bile acid, or protein, between those with and those without gallstones; 2) no negative correlation between D-glucaric acid content and beta-glucuronidase activity in the bile; and 3) minimal conversion of D-glucaric acid to D-glucaro-1,4- lactone at the usual pH of bile. We conclude that biliary D-glucaric acid plays no role in the prevention of gallstone formation.
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Authors | Y D Ho, K J Ho |
Journal | Scandinavian journal of gastroenterology
(Scand J Gastroenterol)
Vol. 25
Issue 6
Pg. 631-40
(Jun 1990)
ISSN: 0036-5521 [Print] England |
PMID | 2359994
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Sugar Acids
- Glucuronidase
- Glucaric Acid
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Topics |
- Bile
(analysis, metabolism)
- Cholelithiasis
(prevention & control)
- Female
- Glucaric Acid
(analysis)
- Glucuronidase
(metabolism)
- Humans
- Male
- Methods
- Sugar Acids
(analysis)
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