Indole-3-carbinol inhibits LPS-induced inflammatory response by blocking TRIF-dependent signaling pathway in macrophages.

Abstract

Indole-3-carbinol (I3C), a natural hydrolysis product of glucobrassicin, is a member of the Brassica family of vegetables and is known to have various anti-cancer activities. In the present study, we assessed in vitro and in vivo anti-inflammatory effects of I3C and its molecular mechanisms. I3C attenuated the production of pro-inflammatory mediators such as NO, IL-6, and IL-1β in LPS-induced Raw264.7 cells and THP-1 cells through attenuation of the TRIF-dependent signaling pathway. Furthermore, I3C suppressed the infiltration of immune cells into the lung and pro-inflammatory cytokine production such as IL-6, TNF-α in broncho-alveolar lavage fluid (BALF) in the LPS-induced acute lung injury mouse model. I3C also suppressed IL-1β secretion in nigericin treated in vivo model. I3C has potent anti-inflammatory effects through regulating TRIF-dependent signaling pathways, suggesting that I3C may provide a valuable therapeutic strategy in treating various inflammatory diseases.

• Authors: Jun Jiang, Tae Bong Kang, Do Wan Shim, Na Hyun Oh, Tack Joong Kim, Kwang Ho Lee
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 57 Pg. 256-61 (Jul 2013) ISSN: 1873-6351 [Electronic] England
• PMID: 23597448 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Adaptor Proteins, Vesicular Transport
• Anti-Inflammatory Agents, Non-Steroidal
• Cytokines
• Indoles
• Inflammation Mediators
• Lipopolysaccharides
• Myd88 protein, mouse
• Myeloid Differentiation Factor 88
• TICAM-1 protein, mouse
• Nitric Oxide
• indole-3-carbinol

Topics

• Adaptor Proteins, Vesicular Transport (metabolism)
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Bronchoalveolar Lavage Fluid (immunology)
• Cell Line (drug effects)
• Cytokines (metabolism)
• Disease Models, Animal
• Female
• Indoles (pharmacology)
• Inflammation (drug therapy, metabolism, pathology)
• Inflammation Mediators (metabolism)
• Lipopolysaccharides (pharmacology)
• Macrophages (drug effects, immunology, metabolism)
• Mice
• Mice, Inbred BALB C
• Myeloid Differentiation Factor 88 (metabolism)
• Nitric Oxide (metabolism)
• Pneumonia (drug therapy, etiology, pathology)
• Signal Transduction (drug effects)