Cellular uptake and antitumour activity of paclitaxel incorporated into trilaurin-based solid lipid nanoparticles in ovarian cancer.

Abstract

Trilaurin-based solid lipid nanoparticles (TL-SLNs) containing paclitaxel (PTX) were prepared by hot-melt high-pressure homogenisation and subsequent freezing and thawing. PTX-containing TL-SLNs showed 160.0 ± 15.8 nm of mean particle size and -43.9 mV of zeta potential. Scanning electron microscopy also revealed the spherical shape and submicron-size of the TL-SLNs. Differential scanning calorimetry measurement presented melting transition peak of TL in SLNs indicating the solidified state of the core lipid. The prepared TL-SLNs were physically stable without significant particle size changes at 4 °C for 2 months. The amounts of uptake into the human ovarian cancer cells, SKOV-3, were similar between PTX delivered in Cremophor EL-based formulation and TL-SLNs. In vitro and in vivo antitumour activity of PTX in TL-SLNs was comparable to the commercial Cremophor EL-based formulation in SKOV-3. These results suggest that PTX-loaded TL-SLNs have promising potential as an alternative parenteral formulation for PTX.

• Authors: Wenting Xu, Soo-Jeong Lim, Mi-Kyung Lee
• Journal: Journal of microencapsulation (J Microencapsul) Vol. 30 Issue 8 Pg. 755-61 ( 2013) ISSN: 1464-5246 [Electronic] England
• PMID: 23594306 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Drug Carriers
• Lipids
• Triglycerides
• trilaurin
• Paclitaxel

Topics

• Antineoplastic Agents, Phytogenic (administration & dosage, pharmacokinetics, pharmacology)
• Cell Line, Tumor
• Drug Carriers (chemistry)
• Female
• Humans
• Lipids (chemistry)
• Nanoparticles (chemistry)
• Ovarian Neoplasms (drug therapy, pathology)
• Ovary (drug effects, pathology)
• Paclitaxel (administration & dosage, pharmacokinetics, pharmacology)
• Triglycerides (chemistry)