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Protective effect of wogonin on proinflammatory cytokine generation via Jak1/3-STAT1/3 pathway in lipopolysaccharide stimulated BV2 microglial cells.

Abstract
Wogonin is a flavonoid compound which exhibits antioxidation, anti-inflammation, neuroprotection, and antitumorgenesis functions. However, the mechanism of how wogonin reduces proinflammatory cytokine generation in activated microglia is unclear. At present, we found wogonin inhibited lipopolysaccharide (LPS)-/interferon-γ (INF-γ)-induced generation of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Wogonin exhibited parallel inhibition on LPS-/INF-γ-induced expression of IL-6 and TNF-α messenger RNA at the same concentration range. LPS-/INF-γ-induced phosphorylation of signal transduction and transcription 1 and 3 (STAT1/3) were also inhibited by wogonin. Although wogonin expressed only weak inhibitory effect on LPS-/INF-γ-induced phosphorylation of Janus kinase-2 (Jak-2) and tyrosine kinase (Tyk)-2, it significantly attenuated the phosphorylation of Jak-1 and Jak-3. These results indicated that the blockade of IL-6 and TNF-α production by wogonin in LPS-/INF-γ-stimulated BV2 microglial cells was attributed mainly to the interference in Jak-1/-3-STAT1/3 signaling pathway.
AuthorsChung-Hsin Yeh, Hung-Che Shih, Hui-Mei Hong, Shiuan-Shinn Lee, Ming-Ling Yang, Chun-Jung Chen, Yu-Hsiang Kuan
JournalToxicology and industrial health (Toxicol Ind Health) Vol. 31 Issue 10 Pg. 960-6 (Oct 2015) ISSN: 1477-0393 [Electronic] England
PMID23592745 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© The Author(s) 2013.
Chemical References
  • Cytokines
  • Flavanones
  • Lipopolysaccharides
  • STAT Transcription Factors
  • Janus Kinases
  • wogonin
Topics
  • Animals
  • Cell Line
  • Cytokines (analysis, metabolism)
  • Flavanones (pharmacology)
  • Janus Kinases (metabolism)
  • Lipopolysaccharides (toxicity)
  • Mice
  • Microglia (cytology, drug effects)
  • STAT Transcription Factors (metabolism)
  • Signal Transduction (drug effects)

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