Abstract |
Wogonin is a flavonoid compound which exhibits antioxidation, anti- inflammation, neuroprotection, and antitumorgenesis functions. However, the mechanism of how wogonin reduces proinflammatory cytokine generation in activated microglia is unclear. At present, we found wogonin inhibited lipopolysaccharide (LPS)-/ interferon-γ (INF-γ)-induced generation of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Wogonin exhibited parallel inhibition on LPS-/INF-γ-induced expression of IL-6 and TNF-α messenger RNA at the same concentration range. LPS-/INF-γ-induced phosphorylation of signal transduction and transcription 1 and 3 (STAT1/3) were also inhibited by wogonin. Although wogonin expressed only weak inhibitory effect on LPS-/INF-γ-induced phosphorylation of Janus kinase-2 (Jak-2) and tyrosine kinase (Tyk)-2, it significantly attenuated the phosphorylation of Jak-1 and Jak-3. These results indicated that the blockade of IL-6 and TNF-α production by wogonin in LPS-/INF-γ-stimulated BV2 microglial cells was attributed mainly to the interference in Jak-1/-3-STAT1/3 signaling pathway.
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Authors | Chung-Hsin Yeh, Hung-Che Shih, Hui-Mei Hong, Shiuan-Shinn Lee, Ming-Ling Yang, Chun-Jung Chen, Yu-Hsiang Kuan |
Journal | Toxicology and industrial health
(Toxicol Ind Health)
Vol. 31
Issue 10
Pg. 960-6
(Oct 2015)
ISSN: 1477-0393 [Electronic] England |
PMID | 23592745
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © The Author(s) 2013. |
Chemical References |
- Cytokines
- Flavanones
- Lipopolysaccharides
- STAT Transcription Factors
- Janus Kinases
- wogonin
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Topics |
- Animals
- Cell Line
- Cytokines
(analysis, metabolism)
- Flavanones
(pharmacology)
- Janus Kinases
(metabolism)
- Lipopolysaccharides
(toxicity)
- Mice
- Microglia
(cytology, drug effects)
- STAT Transcription Factors
(metabolism)
- Signal Transduction
(drug effects)
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