Boerhaavia diffusa L. attenuates angiotensin II-induced hypertrophy in H9c2 cardiac myoblast cells via modulating oxidative stress and down-regulating NF-κβ and transforming growth factor β1.

Abstract

The present study evaluated the antihypertrophic potential of the ethanolic extract of Boerhaavia diffusa (BDE), a well-known edible cardiotonic plant reported in Ayurveda against angiotensin II-induced hypertrophy in H9c2 cardiac myoblast cells. Markers of hypertrophy such as cell size, protein content and the concentrations of atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) were analysed for the confirmation of hypertrophy induction. Angiotensin II (100 nM) caused an increase in cell volume (69·26 (SD 1·21)%),protein content (48·48 (SD 1·64)%), ANP (81·90 (SD 1·22)%) and BNP (108·57 (SD 1·47)%). BDE treatment significantly reduced cell volume, protein content and the concentrations of ANP and BNP (P#0·05) in H9c2 cells. The activity of various antioxidant enzymes and the concentration of reduced glutathione, which was lowered due to hypertrophy, were increased in BDE-treated cells. The BDE treatment also reduced intracellular reactive oxygen species generation, lipid peroxidation and protein carbonyls in cells. In addition,the expression patterns of NF-kb and transforming growth factor b1 were found to be increased during hypertrophy, and their expressions were reduced on BDE treatment. In vitro chemical assays showed that BDE inhibits angiotensin-converting enzyme and xanthine oxidase in a dose-dependent manner with an estimated 50% effective concentration (EC50) value of 166·12 (SD 2·42) and 60·05 (SD 1·54) mg/ml,respectively. The overall results clearly indicate the therapeutic potential of B. diffusa against cardiac hypertrophy, in addition to its nutritional qualities.

• Authors: A Prathapan, V P Vineetha, P A Abhilash, K G Raghu
• Journal: The British journal of nutrition (Br J Nutr) Vol. 110 Issue 7 Pg. 1201-10 (Oct 2013) ISSN: 1475-2662 [Electronic] England
• PMID: 23591029 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Biomarkers
• Cardiovascular Agents
• NF-kappa B
• Plant Extracts
• Reactive Oxygen Species
• Transforming Growth Factor beta1
• Angiotensin II
• Glutathione

Topics

• Angiotensin II (metabolism)
• Animals
• Antioxidants (metabolism, pharmacology)
• Biomarkers (metabolism)
• Cardiovascular Agents (pharmacology)
• Cell Line
• Down-Regulation
• Glutathione (metabolism)
• Hypertrophy
• Lipid Peroxidation
• Myoblasts, Cardiac (drug effects, metabolism, pathology)
• Myocardium (metabolism, pathology)
• NF-kappa B (metabolism)
• Nyctaginaceae
• Oxidative Stress (drug effects)
• Plant Extracts (pharmacology)
• Protein Carbonylation
• Rats
• Reactive Oxygen Species (metabolism)
• Transforming Growth Factor beta1 (metabolism)