Abstract | BACKGROUND: METHODS: Forty rats were allocated into four groups, where rats in Groups LD (low volume+DMSO) and LN (low volume+NS-398) were given intravenously 2ml DMSO and 8mg/kg NS-398 (a specific COX-2 inhibitor, dissolved in 2ml DMSO) before 4-hour lower tidal volume ventilation (8ml/kg), respectively, while DMSO and NS-398 were administrated in the same manner before 4-hour injurious ventilation (40ml/kg) in Groups HD (high volume+DMSO) and HN (high volume+NS-398). The arachidonic acid metabolites (6-keto prostaglandin F1α, thromboxane B2), inflammatory cytokines ( tumor necrosis factor-α, interleukin-1β, 6, 8) and total protein levels in bronchoalveolar lavage (BAL) fluid and COX-2 mRNA and AQP1 protein expression in lung tissue were detected; water content and lung morphology were also evaluated. RESULTS: Compared to Groups LD and LN, the rats in Groups HD and HN suffered obvious lung morphological changes with higher wet-to-dry weight ratio and lung injury score, and the levels of arachidonic acid metabolites, inflammatory cytokines and total protein in BAL fluid were increased, the expression of COX-2 mRNA was significantly upregulated and AQP1 protein was downregulated in lung tissue (p<0.05). The changes in BAL fluid and the severity of lung injury were attenuated, and AQP1 expression was upregulated in Group HN as compared to HD (p<0.05). CONCLUSIONS: Ventilation with large tidal volume causes inflammatory mediator production and AQP1 downregulation, which could be attenuated by COX-2 inhibition.
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Authors | Li-da Jin, Liang-Rong Wang, Li-Qin Wu, Yuan-Lu Shan, Xi-Yue Zhao, Xiang-Qing Xiong, Jun-Hui Zhou, Li-Na Lin, Lie-Lie Jin |
Journal | International immunopharmacology
(Int Immunopharmacol)
Vol. 16
Issue 2
Pg. 288-95
(Jun 2013)
ISSN: 1878-1705 [Electronic] Netherlands |
PMID | 23587488
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier B.V. All rights reserved. |
Chemical References |
- Aqp1 protein, rat
- Cyclooxygenase 2 Inhibitors
- Cytokines
- Nitrobenzenes
- RNA, Messenger
- Sulfonamides
- N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
- Aquaporin 1
- Cyclooxygenase 2
- Ptgs2 protein, rat
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Topics |
- Animals
- Aquaporin 1
(metabolism)
- Bronchoalveolar Lavage Fluid
(chemistry)
- Cyclooxygenase 2
(genetics)
- Cyclooxygenase 2 Inhibitors
(pharmacology, therapeutic use)
- Cytokines
(metabolism)
- Lung
(drug effects, metabolism, pathology)
- Lung Injury
(drug therapy, etiology, metabolism, pathology)
- Male
- Nitrobenzenes
(pharmacology, therapeutic use)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Respiration, Artificial
(adverse effects)
- Sulfonamides
(pharmacology, therapeutic use)
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