Analysis of glycoprotein-bound carbohydrates from pluripotent embryonal carcinoma cells by pokeweed agglutinin-agarose.

Abstract

Embryoglycan is the high-molecular-weight poly-N-acetyllactosamine characteristically and abundantly present in early embryonic cells. Among lectins reacting with poly-N-acetyllactosamines pokeweed agglutinin (PWA) was found to be most useful in analyzing glycoproteins from HM-1 pluripotent embryonal carcinoma (EC) cells, since virtually all glycoproteins carrying embryoglycan in these cells could be isolated by affinity chromatography on PWA-agarose. Furthermore almost all of embryoglycan from HM-1 cells bound to PWA-agarose. Since PWA-agarose used for the present study was confirmed to bind branched but not linear poly-N-acetyllactosamines, the above result indicated that embryoglycan lacking branched poly-N-acetyllactosaminyl chain was scarcely present in these cells. The same approach was used as a mean to show that embryoglycan with Lotus tetragonolobus agglutinin receptor activity also usually has the branched poly-N-acetyllactosamine structure in EC cells. Glycoprotein fractions from PYS-2 parietal endoderm cells and from STO fibroblasts also bound to PWA-agarose. However, the ratio of PWA binding fraction to the total [14C]galactose-labeled glycoproteins was less in these cells as compared to the value in EC cells, and the poly-N-acetyllactosamines from these cells exhibited lower molecular weights than embryoglycan. These results are consistent with our proposal that the complexity and abundance of poly-N-acetyllactosamines distinguishes EC cells from most other cells.

• Authors: H Muramatsu, T Muramatsu
• Journal: Journal of biochemistry (J Biochem) Vol. 107 Issue 4 Pg. 629-34 (Apr 1990) ISSN: 0021-924X [Print] England
• PMID: 2358437 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycoproteins
• Pokeweed Mitogens
• Polysaccharides
• Receptors, Mitogen
• embryoglycan
• poly-N-acetyllactosamine
• Galactose

Topics

• Animals
• Chromatography, Agarose (methods)
• Embryonal Carcinoma Stem Cells
• Galactose (metabolism)
• Glycoproteins (analysis)
• Methylation
• Mice
• Neoplastic Stem Cells (analysis)
• Pokeweed Mitogens (analysis)
• Polysaccharides (metabolism)
• Receptors, Mitogen (isolation & purification)