MicroRNA-24 inhibits osteosarcoma cell proliferation both in vitro and in vivo by targeting LPAATβ.

Abstract

Lysophosphatidic Acid Acyltransferase β (LPAATβ) may be critically involved in osteosarcoma cell proliferation. However, the comprehensive mechanisms responsible for regulation of LPAATβ in osteosarcoma cells remain unclear. This study found that enhanced LPAATβ expression was correlated with osteosarcoma cell proliferation. MiR-24, targeted to LPAATβ, was down-regulated in osteosarcoma cells. Overexpression of miR-24 down-regulated LPAATβ expression in osteosarcoma cells. Specifically, overexpression of miR-24 inhibited osteosarcoma cell proliferation, however, such effect was blocked when LPAATβ activity was inhibited. In conclusion, our study indicates that miR-24 is reduced in osteosarcoma cells, contributing to up-regulation of LPAATβ and resultant osteosarcoma cell proliferation.

• Authors: Lei Song, Jie Yang, Ping Duan, Jianzhong Xu, Xiangdong Luo, Fei Luo, Zehua Zhang, Tianyong Hou, Bing Liu, Qiang Zhou
• Journal: Archives of biochemistry and biophysics (Arch Biochem Biophys) Vol. 535 Issue 2 Pg. 128-35 (Jul 15 2013) ISSN: 1096-0384 [Electronic] United States
• PMID: 23578572 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• MIRN24 microRNA, human
• MicroRNAs
• RNA, Messenger
• RNA, Small Interfering
• Acyltransferases
• 2-acylglycerophosphate acyltransferase

Topics

• Acyltransferases (genetics, metabolism)
• Animals
• Bone Neoplasms (metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Down-Regulation
• Female
• Humans
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• MicroRNAs (metabolism)
• Neoplasm Transplantation
• Osteosarcoma (metabolism, pathology)
• RNA, Messenger (metabolism)
• RNA, Small Interfering (genetics)
• Transplantation, Heterologous
• Up-Regulation