Association of high levels of α-defensins and S100A proteins with Candida mannan detection in bronchoalveolar lavage fluid of preterm neonates.

Abstract

BACKGROUND:

Candida mannan (Mn) detection in bronchoalveolar lavage fluid (BALF) was shown to be useful for earlier identification and preemptive therapy targeting in preterm infants at high risk of invasive Candida infection. We investigated whether early detection of Candida Mn in BALF is associated with the presence of some neutrophilic products, as markers of prenatal infection/inflammation.

METHODS:

BALF specimens were collected during the first 48 h of life from mechanically ventilated preterm newborns. Samples were analyzed by high-performance liquid chromatography-electrospray ionization-mass spectrometry. The relative amounts of α-defensins 1-4 and S100A proteins were measured by extracted ion current peak area. Absolute and differential white cell counts in BALF were obtained. Mn antigen concentrations were determined by the Platelia Candida antigen kit.

RESULTS:

Twenty-five studied neonates were divided into two groups: Mn-positive group and Mn-negative group. Levels of α-defensins 1-4 and S100A12 were significantly higher in the Mn-positive group than in the Mn-negative group. Moreover, positive significant correlations between the absolute number of neutrophils and the levels of α-defensins 1-4 and S100A8 were observed.

CONCLUSION:

The detection of Mn antigen in BALF of preterm infants is consistent with evidence of an innate immune response in their lungs as demonstrated by higher levels of α-defensins and S100A proteins.

• Authors: Giovanni Vento, Alessandra Lio, Chiara Tirone, Claudia Aurilia, Milena Tana, Andrea Piras, Cinzia Ricci, Sarah Perelli, Costantino Romagnoli, Brunella Posteraro, Federica Iavarone, Tiziana Cabras, Chiara Fanali, Irene Messana, Massimo Castagnola
• Journal: Pediatric research (Pediatr Res) Vol. 74 Issue 1 Pg. 19-25 (Jul 2013) ISSN: 1530-0447 [Electronic] United States
• PMID: 23575874 (Publication Type: Journal Article)

Chemical References

• S100 Proteins
• alpha-Defensins

Topics

• Bronchoalveolar Lavage Fluid (microbiology)
• Candida (isolation & purification)
• Chromatography, High Pressure Liquid
• Female
• Humans
• Infant, Newborn
• Infant, Premature
• Male
• S100 Proteins (metabolism)
• Spectrometry, Mass, Electrospray Ionization
• alpha-Defensins (metabolism)