During pregnancy, Plasmodium falciparum-infected erythrocytes cytoadhere to the placenta.
Infection is likely initiated at two sites where placental trophoblasts contact maternal blood: 1) via syncytiotrophoblast (
STB), a multicellular transporting and biosynthetic layer that forms the surface of chorionic villi and lines the intervillous space, and 2) through invasive cytotrophoblasts, which line uterine vessels that divert blood to the placenta. Here, we investigated mechanisms of infected erythrocyte sequestration in relationship to the microanatomy of the maternal-fetal interface. Histological analyses revealed STB denudation in placental
malaria, which brought the stromal cores of villi in direct contact with maternal blood. STB denudation was associated with
hemozoin deposition (P = 0.01) and leukocyte infiltration (P = 0.001) and appeared to be a feature of chronic placental
malaria. Immunolocalization of infected red blood cell receptors (CD36, ICAM1/CD54, and
chondroitin sulfate A) in placentas from uncomplicated pregnancies showed that STB did not
stain, while the underlying villous stroma was immunopositive. Invasive cytotrophoblasts expressed ICAM1. In
malaria, STB denudation exposed CD36 and
chondroitin sulfate A in the villous cores to maternal blood, and STB expressed ICAM1. Finally, we investigated infected erythrocyte adherence to novel receptors by screening an array of 377
glycans. Infected erythrocytes bound
Lewis antigens that immunolocalized to STB. Our results suggest that P. falciparum interactions with STB-associated
Lewis antigens could initiate placental
malaria. Subsequent pathologies, which expose CD36, ICAM1, and
chondroitin sulfate A, might propagate the
infection.