Effects of various lysosphingolipids on cell growth, morphology and lipid composition in three neuroblastoma cell lines.

Abstract

The cell numbers of three mouse neuroblastoma cell lines were decreased upon incubation with lysosphingolipids in the following order of effectiveness: lysosulfatide (lysoCS) greater than psychosine (Ps) greater than sphingosylphosphocholine (SPC). The different cell lines showed characteristic sensitivities to various concentrations of lysolipids less than 150 microM. Interestingly, only SPC induced neurite outgrowth and changed the lipid composition, modifying the amounts of cholesterol, sphingomyelin (SM) and ganglioside GM3 in all cell lines. The effect of SPC on these cell lines was comparable to the effect of N-acetyl SPC (NAcSPC) rather than that of SM.

• Authors: E Sugiyama, K Uemura, A Hara, T Taketomi
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 169 Issue 2 Pg. 673-9 (Jun 15 1990) ISSN: 0006-291X [Print] United States
• PMID: 2357225 (Publication Type: Journal Article)

Chemical References

• Gangliosides
• Phospholipids
• sphingosine phosphorylcholine
• Phosphorylcholine
• Psychosine
• psychosine-3'-sulfate ester
• Choline
• Sphingosine

Topics

• Animals
• Cell Division (drug effects)
• Cell Line
• Choline (analogs & derivatives)
• Gangliosides (metabolism)
• Kinetics
• Lipid Metabolism
• Mice
• Neuroblastoma
• Phospholipids (metabolism)
• Phosphorylcholine (analogs & derivatives, pharmacology)
• Psychosine (analogs & derivatives, pharmacology)
• Sphingosine (analogs & derivatives, pharmacology)
• Tumor Cells, Cultured (cytology, drug effects, metabolism)