Abstract | BACKGROUND: METHODS: A total of 114 OSCC biopsies and corresponding normal tissue were collected at Groote Schuur Hospital and Tygerberg Hospital, Cape Town (South Africa), that were subjected to RNA and DNA isolation. RNA samples were analysed by quantitative Light Cycler RT-PCR for the expression of selected genes involved in inflammation and infection, while conventional PCR was performed on the DNA samples to assess the presence of integrated viral DNA. Further, an in vitro infection assay using HPV pseudovirions was established to study the influence of inflammation on viral infectivity using selected cell lines. RESULTS: HPV DNA was found in about 9% of OSCC patients, comprising predominantly the oncogenic type HPV18. The inflammatory markers IL6 and IL8 as well as the potential HPV receptor ITGA6 were significantly elevated while IL12A was downregulated in the tumour tissues. However, none of these genes were expressed in a virus-dependent manner. When inflammation was mimicked with various inflammatory stimulants such as benzo-α- pyrene, lipopolysaccharide and peptidoglycan in oesophageal epithelial cell lines in vitro, HPV18 pseudovirion uptake was enhanced only in the benzo-α- pyrene treated cells. Interestingly, HPV pseudovirion infectivity was independent of the presence of the ITGA6 receptor on the surface of the tested cells. CONCLUSION: This study showed that although the carcinogen benzo-α- pyrene facilitated HPV pseudovirion uptake into cells in culture, HPV infectivity was independent of inflammation and seems to play only a minor role in oesophageal cancer.
|
Authors | Georgia Schäfer, Siti Kabanda, Beverly van Rooyen, Martina Bergant Marušič, Lawrence Banks, M Iqbal Parker |
Journal | BMC cancer
(BMC Cancer)
Vol. 13
Pg. 185
(Apr 09 2013)
ISSN: 1471-2407 [Electronic] England |
PMID | 23570247
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
|
Topics |
- Alphapapillomavirus
(physiology)
- Carcinoma, Squamous Cell
(virology)
- Cell Line
- Cell Transformation, Viral
- DNA, Viral
- Esophageal Neoplasms
(virology)
- Esophagitis
(pathology, virology)
- Humans
- Mucous Membrane
(pathology, virology)
- Papillomavirus Infections
(virology)
|