Anticancer effect of the extracts from Polyalthia evecta against human hepatoma cell line (HepG2).

Abstract

OBJECTIVE:

To investigate the anticancer activity of Polyalthia evecta (P. evecta) (Pierre) Finet & Gagnep against human hepatoma cell line (HepG2).

METHODS:

The anticancer activity was based on (a) the cytotoxicity against human hepatoma cells (HepG2) assessed using a neutral red assay and (b) apoptosis induction determined by evaluation of nuclei morphological changes after DAPI staining. Preliminary phytochemical analysis of the crude extract was assessed by HPLC analysis.

RESULTS:

The 50% ethanol-water crude leaf extract of P. evecta (EW-L) showed greater potential anticancer activity with high cytotoxicity [IC50 = (62.8 ± 7.3)µg/mL] and higher selectivity in HepG2 cells than normal Vero cells [selective index (SI) = 7.9]. The SI of EW-L was higher than the positive control, melphalan (SI = 1.6) and the apoptotic cells (46.4 ± 2.6) % induced by EW-L was higher than the melphalan (41.6 ± 2.1)% (P<0.05). The HPLC chromatogram of the EW-L revealed the presence of various kinds of polyphenolics and flavonoids in it.

CONCLUSIONS:

P. evecta is a potential plant with anticancer activity. The isolation of pure compounds and determination of the bioactivity of individual compounds will be further performed.

• Authors: Sasipawan Machana, Natthida Weerapreeyakul, Sahapat Barusrux
• Journal: Asian Pacific journal of tropical biomedicine (Asian Pac J Trop Biomed) Vol. 2 Issue 5 Pg. 368-74 (May 2012) ISSN: 2221-1691 [Print] China
• PMID: 23569932 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Plant Extracts

Topics

• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis
• Carcinoma, Hepatocellular (drug therapy)
• Cell Line, Tumor
• Hep G2 Cells (drug effects)
• Humans
• Liver Neoplasms (drug therapy)
• Phytotherapy (methods)
• Plant Extracts (pharmacology)
• Plant Leaves (chemistry)
• Polyalthia (chemistry)