Tyrosol attenuates ischemia-reperfusion-induced kidney injury via inhibition of inducible nitric oxide synthase.

Abstract

Tyrosol is a natural phenolic antioxidant compound. Oxidative stress represents one of the important mechanisms underlying ischemia-reperfusion-induced kidney injury. The aim of this study was to investigate the effect of tyrosol against ischemia-reperfusion-induced acute kidney injury. The left kidney of Sprague-Dawley rats was subjected to 45 min of ischemia followed by reperfusion for 6 h. Ischemia-reperfusion caused an increase in peroxynitrite formation and lipid peroxidation. The level of nitric oxide (NO) metabolites and the mRNA of inducible nitric oxide synthase (iNOS) were elevated in ischemia-reperfused kidneys. Administration of tyrosol (100 mg/kg body weight) to rats prior to the induction of ischemia significantly reduced peroxynitrite formation, lipid peroxidation, and the level of NO metabolites. Tyrosol administration also attenuated ischemia-reperfusion-induced NF-κB activation and iNOS expression. Such a treatment improved kidney function. Results suggest that tyrosol may have a protective effect against acute kidney injury through inhibition of iNOS-mediated oxidative stress.

• Authors: Pengqi Wang, Qingjun Zhu, Nan Wu, Yaw L Siow, Harold Aukema, Karmin O
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 61 Issue 15 Pg. 3669-75 (Apr 17 2013) ISSN: 1520-5118 [Electronic] United States
• PMID: 23566115 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• RNA, Messenger
• 4-hydroxyphenylethanol
• Nitric Oxide
• Nitric Oxide Synthase Type II
• Nos2 protein, rat
• Phenylethyl Alcohol

Topics

• Animals
• Antioxidants (administration & dosage, therapeutic use)
• Dose-Response Relationship, Drug
• Down-Regulation (drug effects)
• Injections, Intraperitoneal
• Kidney (blood supply, drug effects, metabolism, physiopathology)
• Lipid Peroxidation (drug effects)
• Male
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type II (genetics, metabolism)
• Oxidative Stress (drug effects)
• Phenylethyl Alcohol (administration & dosage, analogs & derivatives, therapeutic use)
• RNA, Messenger (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, physiopathology, prevention & control)