Abstract | BACKGROUND: METHODS: We established a murine model of hepatic steatosis in which liver injury is induced by orally feeding mice a PN solution. C57BL/6J mice were randomized to receive PN alone (a high carbohydrate diet (HCD)), PN plus intravenous FO (Omegaven®; Fresenius Kabi AG, Bad Homburg VDH, Germany), PN plus intravenous SO (Intralipid®; Fresenius Kabi AG, Bad Homburg v.d.H., Germany, for Baxter Healthcare, Deerfield, IL), or a chow diet. After 19 days, liver tissue was harvested from all animals and subjected to metabolomic profiling. RESULTS: The administration of an oral HCD without lipid induced profound hepatic steatosis. SO was associated with macro- and microvesicular hepatic steatosis, while FO largely prevented the development of steatosis. 321 detectable compounds were identified in the metabolomic analysis. HCD induced de novo fatty acid synthesis and oxidative stress. Both FO and SO relieved some of the metabolic shift towards de novo lipogenesis, but FO offered additional advantages in terms of lipid peroxidation and the generation of inflammatory precursors. CONCLUSIONS: Improved lipid metabolism combined with reduced oxidative stress may explain the protective effect offered by intravenous FO in vivo.
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Authors | Brian T Kalish, Hau D Le, Kathleen M Gura, Bruce R Bistrian, Mark Puder |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 4
Pg. e59653
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 23565157
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antioxidants
- Fat Emulsions, Intravenous
- Fatty Acids
- Fatty Acids, Essential
- Membrane Lipids
- Parenteral Nutrition Solutions
- Phospholipids
- Triglycerides
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Topics |
- Animals
- Antioxidants
(metabolism)
- Disease Models, Animal
- Fat Emulsions, Intravenous
(adverse effects)
- Fatty Acids
(biosynthesis)
- Fatty Acids, Essential
(metabolism)
- Fatty Liver
(etiology, metabolism, pathology)
- Lipid Peroxidation
- Male
- Membrane Lipids
(metabolism)
- Metabolome
- Metabolomics
- Mice
- Oxidative Stress
- Parenteral Nutrition Solutions
(adverse effects)
- Phospholipids
(metabolism)
- Triglycerides
(biosynthesis)
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