Abstract | OBJECTIVE: STUDY DESIGN: Experimental, in vitro. SETTING: SUBJECTS AND METHODS: A panel of stage- and anatomic-site specific primary and metastatic HNSCC cell lines was assessed by flow cytometry to quantify sLeX relative expression levels. Serum-free conditioned media from the same HNSCC lines was collected over a time course of 72 hours and assessed by Western blot for secreted sLeX expression. Representative HNSCC cell lines were cultured as floating orospheres (condition that enhance CSC growth) or under normal adherent conditions and characterized by flow cytometry for CSC markers (CD44, aldehyde dehydrogenase [ALDH]) comparatively with sLeX expression. RESULTS: sLeX is predominantly expressed in carcinomas originating from the oral cavity. Secreted sLeX is also found to be high in oral carcinomas and increased over the analyzed time course. Floating orospheres were strongly positive for CD44 and ALDH, confirming CSC enrichment of the orospheres. Tumor cells grown as orospheres are 95% to 100% positive for sLeX compared to 10% to 40% of adherent counterpart. CONCLUSION: These studies provide the first evidence of sLeX relationship with CSC in HNSCC.
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Authors | Michael J Czerwinski, Vincenzo Desiderio, Omar Shkeir, Petros Papagerakis, Martian C Lapadatescu, John H Owen, Maria Athanassiou-Papaefthymiou, Li Zheng, Gianpaolo Papaccio, Mark E Prince, Silvana Papagerakis |
Journal | Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery
(Otolaryngol Head Neck Surg)
Vol. 149
Issue 1
Pg. 97-104
(Jul 2013)
ISSN: 1097-6817 [Electronic] England |
PMID | 23558285
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Hyaluronan Receptors
- Oligosaccharides
- Sialyl Lewis X Antigen
- Aldehyde Dehydrogenase
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Topics |
- Aldehyde Dehydrogenase
(physiology)
- Carcinoma, Squamous Cell
(metabolism, pathology)
- Cell Culture Techniques
- Cell Line, Tumor
- Head and Neck Neoplasms
(metabolism, pathology)
- Humans
- Hyaluronan Receptors
(physiology)
- Neoplastic Stem Cells
(metabolism, pathology)
- Oligosaccharides
(metabolism)
- Sialyl Lewis X Antigen
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