Fifty percent tissue culture infective dose assay for determining the titer of infectious human herpesvirus 8.

We have developed a human herpesvirus 8 (HHV-8) 50% tissue culture infective dose (TCID50) assay using the T1H6-DC-SIGN cell line. Infection of T1H6-DC-SIGN cells with HHV-8 induces expression of β-galactosidase, which was used to determine TCID50 levels. Validation of TCID50 values was performed by immunofluorescence assay of HHV-8 infection of immature dendritic cells at various TCID50 doses.
AuthorsSagar V Nadgir, Heather R Hensler, Emilee R Knowlton, Charles R Rinaldo, Giovanna Rappocciolo, Frank J Jenkins
JournalJournal of clinical microbiology (J Clin Microbiol) Vol. 51 Issue 6 Pg. 1931-4 (Jun 2013) ISSN: 1098-660X [Electronic] United States
PMID23554189 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Validation Studies)
Chemical References
  • beta-Galactosidase
  • Cell Line
  • Dendritic Cells (virology)
  • Genes, Reporter
  • Herpesvirus 8, Human (isolation & purification, pathogenicity, physiology)
  • Humans
  • Viral Load (methods)
  • beta-Galactosidase (analysis)

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