HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Effect of bixin and norbixin on the expression of cytochrome P450 in HepG2 cell line.

Abstract
Bixin and norbixin are the main components of annatto, which is extracted from Bixa orellana and largely used as natural colorant in the food and pharmaceutical industries. Annatto can enhance CYP1A and CYP2B activity in rats; however, the inducer effect has not been investigated in human cell lines. In this study, the ability of bixin and norbixin to induce the cytochrome P450 (CYP) enzymes was assessed in HepG2 human hepatoma cell line. HepG2 cells were treated with bixin and norbixin, and the expression of the CYP genes quantified by real-time reverse transcription polymerase chain reaction (RT-PCR). Expression of CYP1A1 and CYP1A2 was significantly increased by bixin treatment, while CYP2B6, 2C9, 2E1 and 3A4 were unaffected. Cells were treated with norbixin showed no inducer effect. The results suggest that the inducer potential of annatto is attributed to bixin, but not to norbixin, despite their similarities in molecular structure.
AuthorsMíriam Cristina Sakuragui Matuo, Rafael Teruiti de Oliveira Takamoto, Irene Satiko Kikuchi, Terezinha de Jesus Andreoli Pinto
JournalCell biology international (Cell Biol Int) Vol. 37 Issue 8 Pg. 843-8 (Aug 2013) ISSN: 1095-8355 [Electronic] England
PMID23554079 (Publication Type: Journal Article)
Copyright© 2013 International Federation for Cell Biology.
Chemical References
  • Carotenoids
  • Cytochrome P-450 Enzyme System
  • bixin
  • norbixin
Topics
  • Carotenoids (pharmacology)
  • Cell Survival (drug effects)
  • Cytochrome P-450 Enzyme System (genetics, metabolism)
  • Enzyme Induction (drug effects)
  • Gene Expression (drug effects)
  • Hep G2 Cells
  • Humans
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: