Clinical utility of measuring expression levels of KAP1, TIMP1 and STC2 in peripheral blood of patients with gastric cancer.

Abstract

BACKGROUND:

We examined preoperative kinesin II-associated protein (KAP1), TIMP metallopeptidase inhibitor 1 (TIMP1) and stanniocalcin 2 (STC2) expression levels in patients with gastric cancers to assess their clinical application for diagnosing and monitoring diseases.

METHODS:

Real-time reverse transcription-polymerase chain reaction was used to detect the expression levels of KAP1, TIMP1, STC2, talin 2 (TLN2), sushi-repeat-containing protein, X-linked 2 (SRPX2) and secreted protein, acidic, cysteine-rich (SPARC) in the patients' peripheral blood karyocytes. The data were analyzed with receiver operating characteristics (ROC) curves.

RESULTS:

A total of 112 patients with gastric cancer, 42 patients with recurrence and 107 healthy volunteers were recruited. There were significant correlations between KAP1, TIMP1 and STC2 levels, and TNM tumor stages and distant metastases. The area under the ROC curves (AUC) of KAP1 was 0.803 ± 0.040 (P = 0.0001), the AUC of TIMP1 was 0.767 ± 0.043 (P = 0.0001) and the AUC of STC2 was 0.769 ± 0.045 (P = 0.0001), thus differentiating preoperative gastric cancer patients from healthy volunteers by ROC curve analysis. The AUC of STC2 was 0.739 ± 0.070 (P = 0.004) and the AUC of KAP1 was 0.418 ± 0.088 (P = 0.319), thus differentiating recurrence of gastric cancer from healthy volunteers by ROC curve analysis. High TIMP1 and STC2 expression levels were suspected to be poor prognostic factors of disease recurrence in patients with gastric cancer.

CONCLUSIONS:

KAP1, TIMP1 and STC2 expression levels may be potential biomarkers for the screening, diagnosis, prognosis and surveillance of gastric cancer.

• Authors: Yuan-Yu Wang, Li Li, Zhong-Sheng Zhao, Hui-Ju Wang
• Journal: World journal of surgical oncology (World J Surg Oncol) Vol. 11 Pg. 81 (Apr 02 2013) ISSN: 1477-7819 [Electronic] England
• PMID: 23548070 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Glycoproteins
• Intercellular Signaling Peptides and Proteins
• RNA, Messenger
• Repressor Proteins
• STC2 protein, human
• TIMP1 protein, human
• Tissue Inhibitor of Metalloproteinase-1
• TRIM28 protein, human
• Tripartite Motif-Containing Protein 28

Topics

• Adenocarcinoma (blood, mortality, pathology, surgery)
• Adult
• Aged
• Aged, 80 and over
• Area Under Curve
• Biomarkers, Tumor (blood, genetics)
• Case-Control Studies
• Female
• Follow-Up Studies
• Gastrectomy
• Gastric Mucosa (metabolism)
• Glycoproteins (blood, genetics)
• Humans
• Intercellular Signaling Peptides and Proteins (blood, genetics)
• Lymphatic Metastasis
• Male
• Middle Aged
• Neoplasm Recurrence, Local (blood, mortality, pathology, surgery)
• Neoplasm Staging
• Prognosis
• RNA, Messenger (genetics)
• ROC Curve
• Real-Time Polymerase Chain Reaction
• Repressor Proteins (blood, genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Stomach Neoplasms (blood, mortality, pathology, surgery)
• Survival Rate
• Tissue Inhibitor of Metalloproteinase-1 (blood, genetics)
• Tripartite Motif-Containing Protein 28