Abstract | BACKGROUND CONTEXT: Astronauts experience back pain, particularly low back pain, during and after spaceflight. Recent studies have described histologic and biochemical changes in rat intervertebral discs after space travel, but there is still no in vitro model to investigate the effects of microgravity on disc metabolism. PURPOSE: To study the effects of microgravity on disc degeneration and establish an in vitro simulated microgravity study model. STUDY DESIGN: METHODS: The mice discs were cultured in a rotating wall vessel bioreactor where the microgravity condition was simulated. Intervertebral discs were cultured in static and microgravity condition. Histology, biochemistry, and immunohistochemical assays were performed to evaluate the characteristics of the discs in microgravity condition. RESULTS: CONCLUSIONS: We conclude that simulated microgravity induces the molecular changes of disc degeneration. The rotating bioreactor model will provide a foundation to investigate the effects of microgravity on disc metabolism.
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Authors | Li Jin, Gang Feng, Davis L Reames, Adam L Shimer, Francis H Shen, Xudong Li |
Journal | The spine journal : official journal of the North American Spine Society
(Spine J)
Vol. 13
Issue 3
Pg. 235-42
(Mar 2013)
ISSN: 1878-1632 [Electronic] United States |
PMID | 23537452
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Published by Elsevier Inc. |
Chemical References |
- Glycosaminoglycans
- Collagen
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Topics |
- Animals
- Collagen
(metabolism)
- Disease Models, Animal
- Glycosaminoglycans
(metabolism)
- Intervertebral Disc
(metabolism, pathology)
- Intervertebral Disc Degeneration
(metabolism, pathology)
- Lumbar Vertebrae
- Mice
- Tissue Culture Techniques
- Weightlessness Simulation
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