Abstract | BACKGROUND: DESIGN: Twenty-five HIV-infected men with lipodystrophy (LD) and 15 age-matched healthy controls, received an oral glucose tolerance test and a euglycemic-hyperinsulinemic clamp (50 mU/m2/min) combined with 6,6-H2 glucose infusion. Muscle biopsies were obtained and FGF-21 mRNA and glycogen synthase (GS) activity were measured. RESULTS: Subjects with HIV were insulin resistant compared with non-HIV subjects. Compared to controls, HIV subjects demonstrated a twofold increase of plasma FGF-21 from 70.4±56.8 pg/ml vs 109.1±71.8 pg/ml, respectively (p = 0.04) and an eight-fold increase in muscular FGF-21 mRNA expression (p = 0.001). Muscle FGF-21 mRNA correlated inversely with the rate of disappearance of glucose during insulin clamp (r = -0.54, p = 0.0009), and the GS fractional velocity in muscle (r = -0.39, p = 0.03), and directly with fasting insulin (r = 0.50, p = 0.0022), HOMA-IR (r = 0.47, p = 0.004), triglycerides (r = 0.60. P = 0.0001), waist-to-hip ratio (r = 0.51, p = 0.0001) and limb fat mass (-0.46, p = 0.004), but not to plasma FGF-21. CONCLUSION:
FGF-21 mRNA is increased in skeletal muscle in HIV patients and correlates to whole-body (primarily reflecting muscle) insulin resistance, but not to plasma FGF-21. Those findings add to the evidence that FGF-21 is a myokine and may suggest that muscle FGF-21 is working in a local manner.
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Authors | Birgitte Lindegaard, Thine Hvid, Thomas Grøndahl, Christian Frosig, Jan Gerstoft, Pernille Hojman, Bente Klarlund Pedersen |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 3
Pg. e55632
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 23533568
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- fibroblast growth factor 21
- Fibroblast Growth Factors
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Topics |
- Adult
- Case-Control Studies
- Female
- Fibroblast Growth Factors
(blood, genetics, metabolism)
- HIV Infections
(blood, genetics, metabolism)
- Humans
- Insulin Resistance
(genetics, physiology)
- Lipodystrophy
(blood, genetics, metabolism)
- Male
- Middle Aged
- Muscle, Skeletal
(metabolism)
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