Abstract |
The effective antitumorigenic potential of nonsteroidal anti-inflammatory drugs ( NSAIDs) and eicosonoid (EP; EP1-4) receptor antagonists prompted us to test their efficacy in Kaposi's sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV) related lymphomas. Our study demonstrated that (1) EP1-4 receptor protein levels vary among the various non-Hodgkin's lymphoma (NHL) cell lines tested (BCBL-1:KSHV+/EBV-;BC-3: KSHV+/EBV-; Akata/EBV+: KSHV-/EBV+; and JSC-1 cells: KSHV+/EBV + cells); (2) 5.0 μM of EP1 antagonist (SC-51322) had a significant antiproliferative effect on BCBL-1, BC-3, Akata/EBV+, and JSC-1 cells; (3) 50.0 μM of EP2 antagonist ( AH6809) was required to induce a significant antiproliferative effect on BCBL-1, Akata/EBV+, and JSC-1 cells; (4) 5.0 μM of EP4 antagonist (GW 627368X) had a significant antiproliferative effect on BC-3, Akata/EBV+, and JSC-1 cells; (5) COX-2 selective inhibitor celecoxib (5.0 μM) had significant antiproliferative effects on BCBL-1, BC-3, Akata/EBV+, and JSC-1 cells; and (6) a combination of 1.0 μM each of celecoxib, SC-51322 and GW 627368X could potentiate the proapoptotic properties of celecoxib or vice-versa. Overall, our studies identified the synergistic antiproliferative effect of NSAIDs and EP receptor blockers on KSHV and EBV related B cell malignancies.
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Authors | Arun George Paul, Bala Chandran, Neelam Sharma-Walia |
Journal | Translational research : the journal of laboratory and clinical medicine
(Transl Res)
Vol. 161
Issue 6
Pg. 447-68
(Jun 2013)
ISSN: 1878-1810 [Electronic] United States |
PMID | 23523954
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2013 Mosby, Inc. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Antineoplastic Agents
- Cyclooxygenase Inhibitors
- Pyrazoles
- Receptors, Eicosanoid
- Sulfonamides
- Cyclooxygenase 2
- PTGS2 protein, human
- Celecoxib
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Topics |
- Anti-Inflammatory Agents, Non-Steroidal
(pharmacology)
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Celecoxib
- Cell Line, Tumor
- Cell Proliferation
- Cell Survival
(drug effects)
- Cyclooxygenase 2
(metabolism)
- Cyclooxygenase Inhibitors
(pharmacology)
- Drug Screening Assays, Antitumor
- Drug Synergism
- Herpesviridae Infections
(drug therapy, metabolism, virology)
- Herpesvirus 4, Human
(pathogenicity)
- Herpesvirus 8, Human
(pathogenicity)
- Humans
- Lymphoma, B-Cell
(metabolism, pathology, virology)
- Molecular Targeted Therapy
(methods)
- Pyrazoles
(pharmacology)
- Receptors, Eicosanoid
(antagonists & inhibitors, metabolism)
- Sulfonamides
(pharmacology)
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