Abstract | OBJECTIVE: METHODS: Fifty 8-month-old Balb/C mice were divided into five groups. Group 1 received orally the ethanolic extract of propolis (100mg/kg per day) for 7 days, group 2 received intravenously Candida albicans (C. albicans) (2 × 10(5) cell) and orally the ethanolic extract of propolis, and group 3 received intravenously C. albicans. After 18 days of experiments, all mice were euthanized. Then, blood samples were collected, and the spleens were excised. Splenocytes were isolated immediately and cultured in RPMI-1640 medium without stimulation and/or stimulated in the presence of Concanavalin A (Con A) for 48 h. The supernatant of splenocyte cultures and the sera of mice were tested for cytokines such as TNF-α, IFN-γ and IL2 by Enzyme-linked immunosorbent assay (Elisa). RESULTS: Orally-administered propolis treatments showed that it alone suppressed all cytokines understudy (TNF-α, IFN-γ and IL2) in the sera of mice when compared to controls, whereas the cytokine production was strongly stimulated in old mice receiving propolis altogether with C. albicans. CONCLUSION:
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Authors | M Fatahinia, A R Khosravi, H Shokri |
Journal | Journal de mycologie medicale
(J Mycol Med)
Vol. 22
Issue 3
Pg. 237-42
(Sep 2012)
ISSN: 1773-0449 [Electronic] France |
PMID | 23518081
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier Masson SAS. All rights reserved. |
Chemical References |
- Immunologic Factors
- Interleukin-2
- Tumor Necrosis Factor-alpha
- Concanavalin A
- Interferon-gamma
- Propolis
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Topics |
- Administration, Oral
- Aging
(immunology)
- Animals
- Candidiasis, Invasive
(diagnosis)
- Concanavalin A
(pharmacology)
- Drug Evaluation, Preclinical
- Gene Expression Regulation
(drug effects)
- Immunologic Factors
(administration & dosage, pharmacology, therapeutic use)
- Infusions, Intravenous
- Interferon-gamma
(biosynthesis, genetics)
- Interleukin-2
(biosynthesis, genetics)
- Lymphocyte Activation
(drug effects)
- Lymphocytes
(drug effects, immunology)
- Macrophages
(immunology)
- Mice
- Mice, Inbred BALB C
- Propolis
(administration & dosage, pharmacology, therapeutic use)
- Random Allocation
- Spleen
(immunology)
- Tumor Necrosis Factor-alpha
(biosynthesis, genetics)
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