The effect of temperature of eye irrigation solution to reduce corneal endothelial cell loss during phacoemulsification: an in vitro model study.

Abstract

OBJECTIVE:

To compare the efficiency of eye irrigation solution (balanced salt solution: BSS) stored in a refrigerator and air-conditioned room to reduce the number of corneal endothelial cell loss during the process of phacoemulsification.

MATERIAL AND METHOD:

Porcine corneal endothelial cells (PCEC) isolated from porcine eyes were used as a model. The porcine eyes were obtained from a local slaughterhouse within 6 h after death and PCEC were cultured as standard method. The percentage of cell survival was evaluated by MTT assay.

RESULTS:

The PCEC was successfully grown in the standard culturing system. The growth curve of PCEC demonstrated that log phase was reached in 3-6 days. The evaluation of heat effect (40-60 degrees C) to PCEC survival was shown that cells death was found at 55 and 60 degrees C which the percentage of cell survival reduced to 30% at 60 degrees C, 180 sec. Further observation on the efficiency of BSS stored in different conditions, refrigeration (8 degrees C) and air-conditioned room (25 degrees C) to PCEC survival was revealed that the number of cell survival increased from 40% to almost 70% when using refrigerated BSS as irrigation solution, while from air-conditioned BSS only at 55%.

CONCLUSION:

From using PCEC as a model and mimic the process of irrigation in phacoemulsification, it was found that the eye irrigation solution (balanced salt solution: BSS) stored in the refrigerator had a better result in helping protection the corneal endothelial cell loss from heat damaged than BSS stored in air-conditioned room.

• Authors: Wanlaya Uthaisang-Tanechpongtamb, Wiroj Limtrakarn, Somporn Reepolmaha
• Journal: Journal of the Medical Association of Thailand = Chotmaihet thangphaet (J Med Assoc Thai) Vol. 95 Suppl 12 Pg. S83-9 (Dec 2012) ISSN: 0125-2208 [Print] Thailand
• PMID: 23513470 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ophthalmic Solutions

Topics

• Analysis of Variance
• Animals
• Cell Death
• Cell Survival
• Cells, Cultured
• Corneal Endothelial Cell Loss (prevention & control)
• Endothelium, Corneal (drug effects)
• In Vitro Techniques
• Ophthalmic Solutions (pharmacology)
• Phacoemulsification
• Swine
• Temperature
• Therapeutic Irrigation